Emergence of IncX3 Plasmid-Harboring blaNDM-5 Dominated by Escherichia coli ST48 in a Goose Farm in Jiangsu, China
Emergence of IncX3 Plasmid-Harboring blaNDM-5 Dominated by Escherichia coli ST48 in a Goose Farm in Jiangsu, China
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中国江苏养鹅场出现以大肠杆菌 ST48 为主的含有 bla(NDM)(-5) 的 IncX3 质粒
DOI：
10.3389/fmicb.2019.02002
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发表时间：
2019-09-04
影响因子：
5.2
通讯作者：
Wang, Zhiqiang
中科院分区：
文献类型：
--
作者：
Liu, Ziyi;Xiao, Xia;Wang, Zhiqiang
从江苏省鹅场分离到12株碳青霉烯类耐药大肠杆菌。这些分离株对多种抗生素具有耐药性，并且对bla（NDM- 5）呈阳性。所有由bla（NDM-5）介导的碳青霉烯类耐药菌株均成功地与E. coli J53。S1-PFGE和WGS结果表明，bla（NDM-5）定位在IncX 3接合质粒上，大小约为1000 bp。46 kb。所有携带bla（NDM-5）的IncX 3质粒共享相同的遗传背景，几乎与印度和中国分别报道的pNDM_MGR194-bla（NDM- 5）和pNDM-QD 28-bla（NDM- 5）相同。12株菌株分属于3种ST，其中优势型为E.从种鹅场分离的携带bla（NDM- 5）的大肠杆菌为ST 48。对鹅群中bla（NDM- 5）菌株的出现及E.在种鹅场发现的大肠杆菌个案，突显水禽养殖系统内可能存有碳青霉烯酶基因，可能进一步污染环境，威胁公众健康。全面监测鹅养殖场中的碳青霉烯类耐药菌需要进一步研究以评估潜在风险。
Twelve carbapenem-resistant Escherichia coil strains were obtained from goose farms in Jiangsu, China. These isolates were resistant to multiple antimicrobials, and positive for the bla(NDM- 5). The carbapenem-resistance of all strains mediated by bla(NDM-5) were successfully conjugated to E. coli J53. S1-PFGE and WGS results showed bla(NDM-5) was located on IncX3 conjugative plasmids with a size of ca. 46 kb. All bla(NDM-5)-bearing IncX3 plasmids shared the same genetic context almost identical to pNDM_MGR194-bla(NDM- 5)and pNDM-QD28-bla(NDM- 5) reported in India and China, respectively. The twelve strains belonged to three STs, in which the dominant type of E. coli isolated from breeding goose farm carrying bla(NDM- 5) was ST48. The emergence of bla(NDM- 5)-bearing strains in goose farms and the clonal transmission of E. coli within the breeding goose farm highlighted the potential reservoir of carbapenemase genes in waterfowl farming system, which may further contaminate environments and pose a threat to public health. Comprehensive surveillance of carbapenem-resistant bacteria in goose farms warrants further study to evaluate the underlying risks.