Lower GC-content in editing exons: Implications for regulation by molecular characteristics maintained by selection
Lower GC-content in editing exons: Implications for regulation by molecular characteristics maintained by selection
复制标题
编辑外显子中的 GC 含量较低：对通过选择维持的分子特征进行调节的影响。
DOI：
10.1016/j.gene.2008.05.012
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发表时间：
2008-09-15
期刊：
影响因子：
3.5
通讯作者：
Jin, Yongfeng
中科院分区：
文献类型：
--
作者：
Cao, Jun;Wu, Xiaojie;Jin, Yongfeng
我们意外地发现，在果蝇synaptotagmin I转录本中，编辑外显子上的GC 3和GC含量比其他外显子低得多，吉布斯自由能更高，这进一步被其他47个实验验证的样本统计学证实。序列比对，Ks和Ka/Ks分析表明，快速上升的纯化选择发生在编辑外显子，限制核苷酸分歧。编辑外显子中存在特定的分子特征，如较低的GC含量，这意味着一个意想不到的要求，并可能指导RNA编辑的发生。因此，DNA的分子特征、RNA编辑和纯化选择之间可能存在联系。(c)2008 Elsevier B. V.保留所有权利。
We unexpectedly discover that there are much lower GC3 and GC-content and higher Gibbs free energy on editing exons than other exons in the Drosophila synaptotagmin I transcripts, which was further, confirmed statistically by others 47 experimentally-validated samples. Sequence alignment, Ks and Ka/Ks assays suggest that rapidly ascending purifying selection occur in editing exons which constrains nucleotide divergency. The presence of specific molecular characteristics such as lower GC-content in editing exons imply an unexpected requirement and are likely to direct RNA editing occurrence. Thus, relations between molecular characteristics of DNA, RNA editing and purifying selection might be present. (c) 2008 Elsevier B.V. All rights reserved.