Survey of fungi and yeast in polymicrobial infections in chronic wounds
Survey of fungi and yeast in polymicrobial infections in chronic wounds
复制标题
DOI：
10.12968/jowc.2011.20.1.40
复制
发表时间：
2011-01-01
影响因子：
1.9
通讯作者：
Jones, C. E.
中科院分区：
文献类型：
--
作者：
Dowd, S. E.;Hanson, J. Delton;Jones, C. E.
目的：了解慢性创面真菌的发生率、丰度和物种多样性，并描述主要真菌种群的相关性。方法：对915例慢性创面进行全面的分子诊断报告。结果：在915例慢性创面标本中，208例(23%)真菌种类阳性。这些标本在编制的数据集中进一步比较，并细分为四种主要的慢性创面类型(卧位性溃疡、糖尿病足部溃疡、未愈合的外科创面和静脉性小腿溃疡)。然而，弯孢霉、马来色菌、金黄色葡萄球菌、枝孢霉、Ulocladium、Engodontium和Trichtophyton也是这些多菌感染的流行成分。葡萄球菌和念珠菌之间存在显著的细菌/真菌负相关。细菌和真菌属与患者的性别、糖尿病状况和心血管合并症等元数据之间也存在显著的相关性。结论：本次微生物调查显示，真菌是比以前报道的更重要的伤口病原体和机会致病菌，说明了这些未被报道的病原体的影响。随着现代经济高效和全面的分子诊断学的应用，临床医生现在可以通过有针对性的治疗来识别和解决这一慢性创面生物堵塞的重要组成部分，从而改善愈合轨迹。
Objective: To assess the incidence, abundance and species diversity of fungi in chronic wounds, as well as to describe the associations of major fungi populations.Method: Comprehensive molecular diagnostic reports were evaluated from a total of 915 chronic wounds in a retrospective study.Results: Of the 915 clinical specimens, 208 (23%) were positive for fungal species.These samples were further compared in a compiled dataset, and sub-classified among the four major chronic wound types (decubitus ulcer, diabetic foot ulcer, non-healing surgical wound, and venous leg ulcer).The most abundant fungi were yeasts in the genus Candida; however, Curvularia, Malessezia, Aureobasidium, Cladosporium, Ulocladium, Engodontium and Trichtophyton were also found to be prevalent components of these polymicrobial infections. A notable bacterial/fungal negative correlation was found to be apparent between Staphylococcus and Candida. There were also significant relationships between both bacterial and fungal genera and patient metadata including gender, diabetes status and cardiovascular comorbidities.Conclusion: This microbial survey shows that fungi are more important wound pathogens and opportunistic pathogens than previously reported, exemplifying the impact of these under-reported pathogens. With the application of modern cost-effective and comprehensive molecular diagnostics, clinicians can now identify and address this significant component of chronic wound bioburden with targeted therapies, thereby improving healing trajectories.