Development of a real-time PCR assay for detection and quantification of Francisella tularensis.
Development of a real-time PCR assay for detection and quantification of Francisella tularensis.
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开发用于土拉弗朗西斯菌检测和定量的实时 PCR 检测方法。
DOI：
10.7883/yoken.jjid.2006.46
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发表时间：
2006
影响因子：
2.2
通讯作者：
A. Yamada
中科院分区：
文献类型：
--
作者：
O. Fujita;M. Tatsumi;K. Tanabayashi;A. Yamada
兼性胞内细菌，土拉热弗朗西斯菌，是土拉菌病的病原体，也被认为是一个潜在的生物威胁因子，由于其极端的感染性。我们建立了一个实时PCR检测使用LightCycler（LC）系统来检测外膜蛋白（fopA）基因的弗朗西斯菌特异性序列。二十五F对包括16个日本分离株在内的土拉热菌菌株进行LC-PCR测定，结果为阳性，而嗜热弗朗西斯菌和其它细菌物种没有显示出任何特异性荧光信号。使用F.土拉热菌基因组DNA在20 fg和2 ng之间，对应于1.2至1.2 × 10（5）个细菌。新建立的实时荧光PCR技术可用于F.土拉热菌基因组特异性、敏感性和快速性。本试验有助于兔热病实验室诊断的标准化。
The facultative intracellular bacterium, Francisella tularensis, is an etiological agent of tularemia and is also considered to be a potential biological threat agent due to its extreme infectivity. We established a real-time PCR assay using the LightCycler (LC) system to detect a Francisella-specific sequence of the outer membrane protein (fopA) gene. Twenty-five F. tularensis strains including 16 Japanese isolates were subjected to this LC-PCR assay, and were tested positive, whereas Francisella philomiragia and other bacteria species did not show any specific fluorescent signal. A linear response was observed using F. tularensis genomic DNAs of between 20 fg and 2 ng, corresponding to 1.2 to 1.2 x 10(5) bacteria. The newly established real-time PCR allows the detection of the F. tularensis genome specifically, sensitively, and rapidly. This assay may contribute to the standardization of the laboratory diagnosis of tularemia.
影响因子：
2.7
作者：
Wittwer, CT;Ririe, KM;Balis, UJ
通讯作者：
Balis, UJ