Significance of lymphatic invasion on regional lymph node metastasis in early gastric cancer using LYVE-1 immunohistochemical analysis
Significance of lymphatic invasion on regional lymph node metastasis in early gastric cancer using LYVE-1 immunohistochemical analysis
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DOI：
10.1309/ljq9g0x8kp17qxp3
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发表时间：
2007-01-01
影响因子：
3.5
通讯作者：
Ishii, Toshiharu
中科院分区：
文献类型：
--
作者：
Fujimoto, Ai;Ishikawa, Yukio;Ishii, Toshiharu
据报道，淋巴浸润是早期胃癌（EGC）淋巴结转移的预测因子；然而，目前的染色技术无法区分淋巴浸润和血管浸润。我们采用人淋巴管内皮透明质酸受体-1 （LYVE-1）抗体和血管特异性血管性血血病因子（vWF）抗体，明确区分这些血管组织，研究淋巴管侵袭对EGC局部淋巴结转移的意义。从66名淋巴结阳性和66名淋巴结阴性受试者中获得EGC组织，并根据年龄和性别进行匹配。对这些组织进行LYVE-I和vWF抗体免疫染色，多因素logistic回归分析表明，淋巴浸润是区域淋巴结转移的重要独立预测因子（优势比为4.667；P= 0.0094），而血管浸润则不是。因此，通过LYVE-1抗体鉴别淋巴浸润可以预测EGC是否存在区域淋巴结转移。
It has been reported that lymphatic invasion is a predictor for lymph node metastasis in early gastric cancer (EGC); however, it has been impossible to differentiate between lymphatic invasion and blood vessel invasion using current staining techniques. We studied the significance of lymphatic invasion on regional lymph node metastasis in EGC by using human lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) antibody, specific to lymphatic vessels, and von Willebrand factor (vWF) antibody, specific to the blood vessels, to clearly distinguish these vascular tissues.EGC tissues were obtained from 66 node-positive and 66 node-negative subjects and were matched by age and sex. These tissues were immunostained with antibodies against LYVE-I and vWF Multivariate logistic regression analysis demonstrated that lymphatic invasion was a significant independent predictor for regional lymph node metastasis (odds ratio, 4.667; P=.0094), whereas blood vessel invasion was not. Thus, lymphatic invasion identified by LYVE-1 antibody could predict the existence of regional lymph node metastasis in EGC.