A VOLTAGE-DEPENDENT CHLORIDE CURRENT LINKED TO THE CELL-CYCLE IN ASCIDIAN EMBRYOS
A VOLTAGE-DEPENDENT CHLORIDE CURRENT LINKED TO THE CELL-CYCLE IN ASCIDIAN EMBRYOS
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DOI：
10.1126/science.2309122
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发表时间：
1990-03-02
期刊：
影响因子：
56.9
通讯作者：
MOODY, WJ
中科院分区：
文献类型：
--
作者：
BLOCK, ML;MOODY, WJ
在早期海鞘胚胎中发现了电压依赖性氯电流，其在卵母细胞和间期卵裂球中是较小的电导，但在每次细胞分裂期间其幅度瞬时增加超过10倍。在卵裂停滞的胚胎中，无论是精子还是钙离子载体激活的，这种氯电流密度的重复循环都可以记录长达6小时（4个循环）。这些数据表明，细胞周期时钟和该通道的特性之间存在直接联系，这种联系导致早期卵裂球电特性的显著周期性变化。
A voltage-dependent chloride current has been found in early ascidian embryos that is a minor conductance in the oocyte and in interphase blastomeres but that increases transiently in amplitude by more than tenfold during each cell division. Repeated cycles in the density of this chloride current could be recorded for up to 6 hours (four cycles) in cleavage-arrested embryos, whether they were activated by sperm or calcium inophore. These data suggest that there is a direct link between the cell cycle clock and the properties of this channel, a link that results in pronounced cyclical changes in the electrical properties of early blastomeres.