Insulin-like biological activity of culinary and medicinal plant aqueous extracts in vitro
Insulin-like biological activity of culinary and medicinal plant aqueous extracts in vitro
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DOI：
10.1021/jf9904517
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发表时间：
2000-03-01
影响因子：
6.1
通讯作者：
Anderson, RA
中科院分区：
文献类型：
--
作者：
Broadhurst, CL;Polansky, MM;Anderson, RA
为了评估对胰岛素功能的可能影响，使用大鼠附睾脂肪细胞测定法测试了49种草药、香料和药用植物提取物对葡萄糖的胰岛素依赖性利用。肉桂是最具生物活性的产品，其次是金缕梅、绿色和红茶、多香果、月桂叶、肉豆蔻、丁香、蘑菇和啤酒酵母。肉桂、茶叶、金缕梅、丁香、月桂叶和多香果经聚乙烯吡咯烷酮（PVP）处理后，其葡萄糖氧化增强活性均消失，表明这些活性物质可能是酚类物质。鼠尾草、蘑菇和啤酒酵母的活性不被PVP去除。一些产品，如高丽参，亚麻籽粉，罗勒已被报道是有效的抗糖尿病药物，但他们只有轻微的活性，在我们的测定。我们的技术测量细胞葡萄糖代谢的直接刺激，因此可能是这些植物中的活性植物化学物质通过其他机制改善葡萄糖代谢，或者这种体外筛选不是某些产品体内降血糖作用的可靠预测因子。总之，特定植物提取物对胰岛素活性的积极作用表明这些植物在改善葡萄糖和胰岛素代谢中可能起作用。
To evaluate the possible effects on insulin function, 49 herb, spice, and medicinal plant extracts were tested in the insulin-dependent utilization of glucose using a rat epididymal adipocyte assay. Cinnamon was the most bioactive product followed by witch hazel, green and black teas, allspice, bay leaves, nutmeg, cloves, mushrooms, and brewer's yeast. The glucose oxidation enhancing bioactivity was lost from cinnamon, tea, witch hazel, cloves, bay leaf and allspice by poly-vinylpyrrolidone) (PVP) treatment, indicating that the active phytochemicals are likely to be phenolic in nature. The activity of sage, mushrooms, and brewers's yeast was not removed by PVP. Some products such as Korean ginseng, flaxseed meal, and basil have been reported to be effective antidiabetic agents; however, they were only marginally active in our assay. Our technique measures direct stimulation of cellular glucose metabolism, so it may be that the active phytochemicals in these plants improve glucose metabolism via other mechanisms or that this in vitro screening is not a reliable predictor of hypoglycemic effects in vivo for some products. In summary, the positive effects of specific plant extracts on insulin activity suggest a possible role of these plants in improving glucose and insulin metabolism.