Modification of Tobacco Mosaic Virus by Polyornithine and Lecithin
Modification of Tobacco Mosaic Virus by Polyornithine and Lecithin
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聚鸟氨酸和卵磷脂对烟草花叶病毒的修饰
DOI：
10.1111/j.1348-0421.1980.tb02864.x
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发表时间：
1980
影响因子：
2.6
通讯作者：
T. Abe
中科院分区：
文献类型：
--
作者：
Y. Kiho;T. Abe
多鸟氨酸和卵磷脂联合作用修饰烟草花叶病毒（TMV）病毒粒子，使其对核糖核酸酶（RNase）、pronase或Triton X‐100敏感。沉积分析和荧光光谱检测表明，修饰TMV经RNase处理后得到的反应产物是由外壳蛋白、聚鸟氨酸和卵磷脂组成的三组分复合物。十六烷基三甲基溴化铵完全满足了改性的最低要求，表明带正电的氮基和中等大小的烷基C10-18是必要的组分。这些成分与TMV表面区域发生反应，被认为在TMV病毒粒子的外壳蛋白亚基连接中起重要作用。
Combined action of polyornithine and lecithin modified tobacco mosaic virus (TMV) virions making them sensitive to ribonuclease (RNase), pronase or Triton X‐100. Sedimentational analysis and examination of the fluorescence spectrum revealed that the reaction product obtained after RNase treatment of modified TMV was a three‐component complex made of coat protein, polyornithine and lecithin. The minimum requirement for the modification was completely fulfilled by cetyltrimethylammonium bromide, suggesting that a positively charged nitrogen group and an alkyl group of moderate size, C10–18, are necessary components. These components react with the surface region of TMV which is considered to have an important role in connecting coat protein subunits in TMV virions.