Comparison of immunoblotting, calculation of the Goldmann-Witmer coefficient, and real-time PCR using aqueous humor samples for diagnosis of ocular toxoplasmosis
Comparison of immunoblotting, calculation of the Goldmann-Witmer coefficient, and real-time PCR using aqueous humor samples for diagnosis of ocular toxoplasmosis
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DOI：
10.1128/jcm.01900-07
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发表时间：
2008-06-01
影响因子：
9.4
通讯作者：
Paris, L.
中科院分区：
文献类型：
--
作者：
Fekkar, A.;Bodaghi, B.;Paris, L.
比较了眼弓形虫病（OT）的三种生物学诊断方法。通过三种方法分析了34例OT患者和76例其他眼部疾病患者的配对房水和血清样本：免疫印迹或Western印迹（WB），Goldmann-Witmer系数（GWC）的计算和PCR。WB和GWC均显示81%的样本（30/37）眼内产生特异性抗弓形虫免疫球蛋白G。PCR检测弓形体DNA在38%的样品（13 34）。13例PCR阳性患者中有9例具有免疫活性。结合这些技术显著提高了诊断灵敏度，GWC-WB组合为92%，WB-PCR组合为90%，GWC-PCR组合为93%。所有三种技术的组合将灵敏度提高到97%。
We compared three biological methods for the diagnosis of ocular toxoplasmosis (OT). Paired aqueous humor and serum samples from 34 patients with OT and from 76 patients with other ocular disorders were analyzed by three methods: immunoblotting or Western blotting (WB), the calculation of the Goldmann-Witmer coefficient (GWC), and PCR. WB and GWC each revealed the intraocular production of specific anti-Toxoplasma immunoglobulin G in 81% of samples (30 of 37). PCR detected toxoplasmic DNA in 38% of samples (13 of 34). Nine of the 13 PCR-positive patients were immunocompetent. Combining the techniques significantly improved the diagnostic sensitivity, to 92% for the GWC-WB combination, 90% for the WB-PCR combination, and 93% for the GWC-PCR combination. The combination of all three techniques improved the sensitivity to 97%.