IGF2BP3 facilitates cell proliferation and tumorigenesis via modulation of JAK/STAT signalling pathway in human bladder cancer.
IGF2BP3 facilitates cell proliferation and tumorigenesis via modulation of JAK/STAT signalling pathway in human bladder cancer.
复制标题
DOI：
10.1111/jcmm.16003
复制
发表时间：
2020-12
影响因子：
5.3
通讯作者：
Wang G
中科院分区：
文献类型：
--
作者：
Huang W;Li Y;Zhang C;Zha H;Zhou X;Fu B;Guo J;Wang G
据报道，胰岛素样生长因子 2 信使 RNA 结合蛋白 3 (IGF2BP3) 有助于多种人类癌症的肿瘤发生。然而，IGF2BP3 在膀胱癌中的生物学功能尚不清楚。我们研究了IGF2BP3表达与膀胱癌患者预后之间的关系。进行细胞增殖、细胞周期和细胞凋亡测定以评估IGF2BP3功能。结果显示，与正常膀胱组织相比，IGF2BP3在膀胱癌组织中过表达，其高表达与膀胱癌患者不良预后密切相关。 IGF2BP3的过表达显着促进细胞增殖和细胞周期进程并抑制细胞凋亡，而IGF2BP3的敲除显着抑制增殖，促进细胞凋亡并诱导细胞周期停滞在G0/G1期。从机制上讲，我们揭示了 IGF2BP3 促进膀胱癌细胞中 JAK/STAT 通路的激活。此外，JAK/STAT 抑制剂显着阻断了 IGF2BP3 的促肿瘤活性。体内肿瘤生长也通过 IGF2BP3 的敲除而受到抑制。因此，IGF2BP3通过激活JAK/STAT信号通路促进膀胱癌细胞增殖。这些发现表明 IGF2BP3 在人类膀胱癌进展中表现出致癌作用。
Insulin‐like growth factor‐2 messenger RNA‐binding protein 3 (IGF2BP3) has been reported to contribute to tumorigenesis in several human cancers. However, the biological functions of IGF2BP3 in bladder cancer are poorly understood. We investigated the relation between IGF2BP3 expression and prognosis of bladder cancer patients. Cell proliferation, cell cycle and cell apoptosis assays were performed to assess IGF2BP3 functions. The results showed that IGF2BP3 was overexpressed in bladder cancer tissues compared with that in normal bladder tissues, and its higher expression was closely correlated with poor prognosis in bladder cancer patients. Overexpression of IGF2BP3 markedly promoted cell proliferation and cell cycle progression and inhibited cell apoptosis, while knockdown of IGF2BP3 notably suppressed the proliferation, promoted cell apoptosis and induced cell cycle arrest at the G0/G1 phase. Mechanistically, we revealed that IGF2BP3 promotes the activation of the JAK/STAT pathway in bladder cancer cells. Moreover, the JAK/STAT inhibitor dramatically blocked the tumour‐promoting activity of IGF2BP3. Tumour growth in vivo was also suppressed by knocking down of IGF2BP3. Hence, IGF2BP3 facilitated bladder cancer cell proliferation by activating the JAK/STAT signalling pathway. These findings suggest that IGF2BP3 exhibits an oncogenic effect in human bladder cancer progression.
登录
查看更多内容
影响因子：
4.8
作者：
Xiong, Hua;Zhang, Zhi-Gang;Fang, Jing-Yuan
通讯作者：
Fang, Jing-Yuan
影响因子：
51.1
作者：
Jiang, Zhong;Chu, Peigou G.;Wu, Chin-Lee
通讯作者：
Wu, Chin-Lee
IMP3 表达增强激活 NF-kappa B 通路并促进肾细胞癌进展
影响因子：
3.7
作者：
Pei X;Li M;Zhan J;Yu Y;Wei X;Guan L;Aydin H;Elson P;Zhou M;He H;Zhang H
通讯作者：
Zhang H
影响因子：
4.8
作者：
Samanta, Sanjoy;Pursell, Bryan;Mercurio, Arthur M.
通讯作者：
Mercurio, Arthur M.
影响因子：
3.3
作者：
Xu, Haodong;Bourne, Patricia A.;Wang, Hanlin L.
通讯作者：
Wang, Hanlin L.