Effect of Acupuncture on Allergen-Induced Basophil Activation in Patients with Atopic Eczema: A Pilot Trial
Effect of Acupuncture on Allergen-Induced Basophil Activation in Patients with Atopic Eczema: A Pilot Trial
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DOI：
10.1089/acm.2009.0684
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发表时间：
2011-04-01
影响因子：
2.6
通讯作者：
Ollert, Markus
中科院分区：
文献类型：
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作者：
Pfab, Florian;Athanasiadis, Georgios I.;Ollert, Markus
目的与方法：特应性湿疹的关键症状是瘙痒。针灸已被证明对实验性瘙痒有显著效果；然而，针对特应性湿疹临床瘙痒及相关机制的研究却很缺乏。本研究设计为单中心、单盲（观察者）、前瞻性、随机临床初步试验，并包含一个额外的实验部分。在10名特应性湿疹患者中，我们在一项初步试验中研究了针灸治疗（n = 5）与不治疗（n = 5）相比对瘙痒强度以及过敏原刺激（屋尘螨和梯牧草花粉）后体外嗜碱性粒细胞CD63表达的影响。
结果：视觉模拟量表中的平均瘙痒强度在针灸组显著低于对照组，针灸组在第15天与第0天相比为 -25%±26%，在第33天与第0天相比为 -24%±31%；而对照组在第15天与第0天相比为15%±6%，在第33天与第0天相比为29%±9%。从第0天（治疗前）到第15天（5次针灸治疗后）以及第33天（10次针灸治疗后），在不同浓度（5 ng/mL、1 ng/mL、0.5 ng/mL或0.25 ng/mL）的屋尘螨和草花粉过敏原刺激下，针灸组的CD63阳性嗜碱性粒细胞比对照组少。
结论：我们的结果显示，针灸治疗后特应性湿疹患者的瘙痒强度以及体外过敏原诱导的嗜碱性粒细胞活化降低。降低嗜碱性粒细胞活化可作为研究针灸在免疫球蛋白E介导的过敏中的作用机制的又一工具。由于我们的初步试验纳入的患者数量有限，需要进一步的研究来强化这一假设。
Objective and methods: The crucial symptom of atopic eczema is itch. Acupuncture has been shown to exhibit a significant effect on experimental itch; however, studies focusing on clinical itch in atopic eczema and corresponding mechanisms are lacking. The study design was a unicenter, single-blinded (observer), prospective, randomized clinical pilot trial with an additional experimental part. In 10 patients with atopic eczema, we investigated the effect of acupuncture treatment (n = 5) compared to no treatment (n = 5) on itch intensity and in vitro basophil CD63 expression upon allergen stimulation (house dust mite and timothy grass pollen) in a pilot trial.Results: Mean itch intensity in a visual analog scale was rated significantly lower in the acupuncture group (-25% +/- 26% [day 15-day 0]; -24% +/- 31% [day 33-day 0]) than in the control group (15% +/- 6% [day 15-day 0]; 29% +/- 9% [day 33-day 0]). From day 0 (before treatment) to day 15 (after 5 acupuncture treatments) as well as day 33 (after 10 acupuncture treatments), the acupuncture group showed less CD63 positive basophils than the control group regarding stimulation with house dust mite and grass pollen allergen at various concentrations (5 ng/mL, 1 ng/mL, 0.5 ng/mL, or 0.25 ng/mL).Conclusions: Our results show a reduction of itch intensity and of in vitro allergen-induced basophil activation in patients with atopic eczema after acupuncture treatment. Reducing basophil activation can be a further tool in investigating the mechanisms of action of acupuncture in immunoglobulin E-mediated allergy. Due to the limited number of patients included in our pilot trial, further studies are needed to strengthen the hypothesis.