Mutations of acetylcholinesterase which confer insecticide resistance in Drosophila melanogaster populations.
Mutations of acetylcholinesterase which confer insecticide resistance in Drosophila melanogaster populations.
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DOI：
10.1186/1471-2148-4-4
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发表时间：
2004-02-05
影响因子：
3.4
通讯作者：
Fournier D
中科院分区：
文献类型：
--
作者：
Menozzi P;Shi MA;Lougarre A;Tang ZH;Fournier D
有机磷和氨基甲酸酯杀虫剂不可逆地抑制乙酰胆碱酯酶，导致昆虫死亡。抗性修饰的乙酰胆碱酯酶（AChE）已在许多昆虫物种中被描述，并且对其基因的测序允许描述几个点突变。然而，它们的相对频率和制图尚未得到解决。为了分析果蝇乙酰胆碱酯酶中最常见的杀虫剂抗性突变，对从世界不同地区收获的几个菌株中的 Ace 基因进行了克隆和测序。序列比较揭示了四种广泛的突变：I161V、G265A、F330Y 和 G368A。我们在此证实，在同一蛋白质中发现突变是孤立的或组合的，并且我们表明大多数自然群体是异质的，由不同等位基因的混合物组成。突变蛋白的体外表达表明，同一蛋白中的突变组合会产生两个后果：增加耐药水平并提供广泛的耐药性。自然群体中存在多个等位基因，对氨基甲酸酯和有机磷酸酯化合物具有不同的抗性，这将使抗性管理计划的建立变得复杂。
Organophosphate and carbamate insecticides irreversibly inhibit acetylcholinesterase causing death of insects. Resistance-modified acetylcholinesterases(AChEs) have been described in many insect species and sequencing of their genes allowed several point mutations to be described. However, their relative frequency and their cartography had not yet been addressed. To analyze the most frequent mutations providing insecticide resistance in Drosophila melanogaster acetylcholinesterase, the Ace gene was cloned and sequenced in several strains harvested from different parts of the world. Sequence comparison revealed four widespread mutations, I161V, G265A, F330Y and G368A. We confirm here that mutations are found either isolated or in combination in the same protein and we show that most natural populations are heterogeneous, composed of a mixture of different alleles. In vitro expression of mutated proteins showed that combining mutations in the same protein has two consequences: it increases resistance level and provides a wide spectrum of resistance. The presence of several alleles in natural populations, offering various resistance to carbamate and organophosphate compounds will complicate the establishment of resistance management programs.
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DOI：
10.1016/j.bbrc.2003.11.141
发表时间：
2004-01-16
影响因子：
3.1
作者：
Nabeshima, T;Mori, A;Tomita, T
通讯作者：
Tomita, T
影响因子：
56.9
作者：
SMISSAERT, HR
通讯作者：
SMISSAERT, HR
影响因子：
2.6
作者：
Vontas, JG;Hejazi, MJ;Hemingway, J
通讯作者：
Hemingway, J
影响因子：
4.1
作者：
Walsh, SB;Dolden, TA;Williamson, MS
通讯作者：
Williamson, MS
影响因子：
8
作者：
Harel, M;Kryger, G;Sussman, JL
通讯作者：
Sussman, JL