Intra-individual change over time in DNA methylation with familial clustering
Intra-individual change over time in DNA methylation with familial clustering
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DOI：
10.1001/jama.299.24.2877
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发表时间：
2008-06-25
影响因子：
120.7
通讯作者：
Feinberg, Andrew P.
中科院分区：
文献类型：
--
作者：
Bjornsson, Hans T.;Sigurdsson, Martin I.;Feinberg, Andrew P.
随着时间的推移，表观遗传标记（DNA的修饰，如DNA甲基化）的变化可能有助于解释常见人类疾病的晚发性。然而，甲基化或其他表观遗传标记在特定个体中随时间的变化尚未被研究。目的确定个体总体DNA甲基化是否存在纵向变化，并评估甲基化维持是否表现出家族聚类。设计、环境和参与者：我们通过发光甲基化测定（一种基因组范围内DNA甲基化的定量测量方法）测量了全球DNA甲基化，对来自冰岛队列（1991年和2002- 2005年）的111名个体的DNA进行了2次访问，平均间隔11年，对来自犹他州样本（1982- 1985年和1997- 2005年）的126名个体的DNA进行了平均间隔16年的采样。主要结果测量：整体甲基化随时间变化。结果29%的冰岛人甲基化随时间变化大于10% (P
Context Changes over time in epigenetic marks, which are modifications of DNA such as by DNA methylation, may help explain the late onset of common human diseases. However, changes in methylation or other epigenetic marks over time in a given individual have not yet been investigated.Objectives To determine whether there are longitudinal changes in global DNA methylation in individuals and to evaluate whether methylation maintenance demonstrates familial clustering.Design, Setting, and Participants We measured global DNA methylation by luminometric methylation assay, a quantitative measurement of genome- wide DNA methylation, on DNA sampled at 2 visits on average 11 years apart in 111 individuals from an Icelandic cohort ( 1991 and 2002- 2005) and on average 16 years apart in 126 individuals from a Utah sample ( 1982- 1985 and 1997- 2005).Main Outcome Measure Global methylation changes over time.Results Twenty- nine percent of Icelandic individuals showed greater than 10% methylation change over time ( P