Transcriptional analysis of Myceliophthora thermophila on soluble starch and role of regulator AmyR on polysaccharide degradation
Transcriptional analysis of Myceliophthora thermophila on soluble starch and role of regulator AmyR on polysaccharide degradation
复制标题
嗜热毁丝霉对可溶性淀粉的转录分析及调节剂 AmyR 对多糖降解的作用
DOI：
10.1016/j.biortech.2018.05.086
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发表时间：
2018
影响因子：
11.4
通讯作者：
Chaoguang. Tian
中科院分区：
文献类型：
--
作者：
Guanbao Xu;Jingen Li;Qian Liu;Wenliang Sun;Min Jiang;Chaoguang. Tian
嗜热真菌Myceliophthora thermophila具有很强的生物质降解能力，是一种有吸引力的选择，可用作细胞工厂，直接从可再生多糖（如淀粉）而不是单体葡萄糖生产化学品。迄今为止，还没有这种嗜热真菌对淀粉的转录组学分析。本研究确定了M.嗜热菌对可溶性淀粉有反应，342个基因集被鉴定为“淀粉调节子”，包括主要的淀粉分解酶（Mycth_72393）。其过表达导致淀粉酶活性增加35%。此外，在M.嗜热菌能显著提高淀粉酶活性30%。通过CRISPR/Cas9系统缺失amyR导致碳分解代谢物阻遏的缓解和纤维素上木质纤维素酶活性的3倍增加。该研究将促进合理的真菌菌株工程，用于从生物质基质如生玉米淀粉甚至农作物秸秆进行生化生产。
Thermophilic fungusMyceliophthora thermophilahas great capacity for biomass degradation and is an attractive option for use as cell factory to produce chemicals directly from renewable polysaccharides, such as starch, rather than monomer glucose. To date, there has been no transcriptomic analysis of this thermophilic fungus on starch. This study determined the transcriptomic profile ofM. thermophilaresponding to soluble starch and a 342-gene set was identified as a “starch regulon”, including the major amylolytic enzyme (Mycth_72393). Its overexpression led to increased amylase activities on starch by 35%. Furthermore, overexpressing the key amylolytic enzyme regulator AmyR inM. thermophilasignificantly increased amylase activity by 30%. Deletion ofamyRby the CRISPR/Cas9 system led to the relief of carbon catabolite repression and 3-fold increased lignocellulase activities on cellulose. This study will accelerate rational fungal strain engineering for biochemical production from biomass substrates such as raw corn starch and even crop straw.