Preparation of molecularly imprinted polymers for strychnine by precipitation polymerization and multi-step swelling and polymerization and their application for selective extraction of strychnine from nux-vomica extract powder
Preparation of molecularly imprinted polymers for strychnine by precipitation polymerization and multi-step swelling and polymerization and their application for selective extraction of strychnine from nux-vomica extract powder
复制标题
沉淀聚合和多步溶胀聚合制备士的宁分子印迹聚合物及其在马钱子提取粉中选择性提取士的宁的应用
DOI：
10.1002/jssc.201600027
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Hisami Matsunaga and Jun Haginaka
中科院分区：
文献类型：
--
作者：
Yukari Nakamura;Hisami Matsunaga and Jun Haginaka
分别采用沉淀聚合法和多步溶胀聚合法制备了单分散士的宁分子印迹聚合物。在沉淀聚合中，甲基丙烯酸和二乙烯基苯分别用作功能单体和交联剂，而在多步溶胀和聚合中，甲基丙烯酸和乙二醇二甲基丙烯酸酯分别用作功能单体和交联剂。以磷酸钠缓冲液和乙腈为移动的流动相，采用液相色谱法研究了两种方法制备的分子印迹聚合物对士的宁的保留和分子识别性能。除了形状识别，离子和疏水相互作用可以影响保留士的宁在低乙腈含量。两种方法制备的分子印迹聚合物均能选择性识别士的宁。以20 mM磷酸钠缓冲液（pH6.0）/乙腈（50：50，v/v）为移动的流动相，沉淀聚合法制备的分子印迹聚合物对士的宁的保留因子和印迹因子分别为220和58;多步溶胀聚合法制备的分子印迹聚合物对士的宁的保留因子和印迹因子分别为73和4.5。结果表明，沉淀聚合法适合于士的宁分子印迹聚合物的制备。此外，该分子印迹聚合物可成功应用于马钱子浸膏粉中士的宁的选择性提取。
Monodisperse molecularly imprinted polymers for strychnine were prepared by precipitation polymerization and multistep swelling and polymerization, respectively. In precipitation polymerization, methacrylic acid and divinylbenzene were used as a functional monomer and crosslinker, respectively, while in multistep swelling and polymerization, methacrylic acid and ethylene glycol dimethacrylate were used as a functional monomer and crosslinker, respectively. The retention and molecular recognition properties of the molecularly imprinted polymers prepared by both methods for strychnine were evaluated using a mixture of sodium phosphate buffer and acetonitrile as a mobile phase by liquid chromatography. In addition to shape recognition, ionic and hydrophobic interactions could affect the retention of strychnine in low acetonitrile content. Furthermore, molecularly imprinted polymers prepared by both methods could selectively recognize strychnine among solutes tested. The retention factors and imprinting factors of strychnine on the molecularly imprinted polymer prepared by precipitation polymerization were 220 and 58, respectively, using 20 mM sodium phosphate buffer (pH 6.0)/acetonitrile (50:50, v/v) as a mobile phase, and those on the molecularly imprinted polymer prepared by multistep swelling and polymerization were 73 and 4.5. These results indicate that precipitation polymerization is suitable for the preparation of a molecularly imprinted polymer for strychnine. Furthermore, the molecularly imprinted polymer could be successfully applied for selective extraction of strychnine innux‐vomicaextract powder.