Transcription attenuation: a highly conserved regulatory strategy used by bacteria
Transcription attenuation: a highly conserved regulatory strategy used by bacteria
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DOI：
10.1016/j.tig.2005.03.002
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发表时间：
2005-05-01
影响因子：
11.4
通讯作者：
Yanofsky, C
中科院分区：
文献类型：
--
作者：
Merino, E;Yanofsky, C
我们采用了计算分析来评估序列元件的保守性，这些序列元素被认为对于调节细菌中直系同源基因家族的各种转录侵入（终止）机制至关重要。我们搜索了转录衰减器的每个预测转录单元的上游序列。然后，这些结构基因的矫正关系聚集了。发现许多受转录衰减调节的基因家族与具有特定蛋白质，tRNA或小型代谢物的结合位点的调节区域相邻。使用我们的方法，我们预测至少80种不同的直系同源群（COG）受转录衰减显着调节。
We employed computational analyses to assess the conservation of sequence elements that are believed to be essential for the various transcription-attenuation (termination) mechanisms that are used to regulate expression of families of orthologous genes in bacteria. We searched the upstream sequence of every predicted transcription unit for a transcription attenuator. These were then clustered by the orthology relationships of the nearby structural genes. Many gene families regulated by transcription attenuation were found to be adjacent to a regulatory region that had a binding site for a specific protein, tRNA or small metabolite. Using our methodology, we predict that at least 80 different clusters of orthologous groups (COGs) are significantly regulated by transcription attenuation.