Multiple acute temperature stress affects leucocyte populations and antibody responses in common carp, Cyprinus carpio L.
Multiple acute temperature stress affects leucocyte populations and antibody responses in common carp, Cyprinus carpio L.
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DOI：
10.1016/s1050-4648(03)00006-8
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发表时间：
2003-11-01
影响因子：
4.7
通讯作者：
Verburg-van Kemenade, BML
中科院分区：
文献类型：
--
作者：
Engelsma, MY;Hougee, S;Verburg-van Kemenade, BML
压力是导致鱼类对病原体易感性增加的潜在因素。在这项研究中，应激诱导的免疫变化可能有助于降低免疫状态进行了调查。采用环境水温下降3 h，降低9℃作为鲤鱼相对轻度和急性应激模型。该应激源对白细胞种群动态的影响用特异性单克隆抗体测定。在单次或多次冷休克后4小时内，总白细胞群中循环b淋巴细胞的相对数量显著下降。这种减少是可逆的，因为b淋巴细胞数量在24小时内恢复。最有可能的是，b淋巴细胞的重新分配促成了这种现象。在头肾中，b淋巴细胞的相对数量增加。粒细胞数量表现出相反的反应：循环中粒细胞占白细胞总数的百分比几乎翻了一番，而头肾中粒细胞的百分比明显下降。这表明，在体内，轻微的应激源会改变白细胞的分布。应激鲤血液中凋亡淋巴细胞的比例显著高于未应激动物。b淋巴细胞和Ig（-）淋巴样细胞有助于细胞凋亡的增加。用抗糖皮质激素受体多克隆血清标记的血淋巴细胞也显示，除b淋巴细胞外，部分Ig（-）淋巴细胞群糖皮质激素受体阳性。由于b淋巴细胞的分布受到很大影响，我们测定了温度胁迫对t淋巴细胞非依赖性（三硝基苯脂多糖）和t淋巴细胞依赖性（二硝基苯锁眼帽贝血青素）体液抗体反应的影响。对t淋巴细胞非依赖性抗原的一抗反应动力学表明，应激鲤鱼在免疫反应开始时抗体滴度较低，这意味着针对t淋巴细胞非依赖性抗原的抗体反应发展较慢。(C) 2003 Elsevier Ltd.版权所有。
Stress is a potential factor causing increased susceptibility of fish to pathogens. In this study, stress-induced immunological changes that may contribute to a decreased immune status were investigated. A 3 h drop in ambient water temperature of 9 degreesC was used as a relative mild and acute stress model for carp. Effects of this stressor on the dynamics of leucocyte populations were determined with specific monoclonal antibodies. The relative number of circulating B-lymphocytes in the total leucocyte population decreased significantly within 4 h after the onset of single or multiple cold shocks. This decrease was reversible, as B-lymphocyte numbers were restored within 24 h. Most probably, a redistribution of B-lymphocytes contributed to this phenomenon. In head kidney, an increase was measured in the relative number of B-lymphocytes. Granulocyte numbers showed opposite reactions: the percentage of granulocytes in the total leucocyte population nearly doubled in circulation and decreased significantly in the head kidney. This demonstrates that in vivo, a mild stressor differentially alters the distribution of leucocytes. In stressed carp, the percentage of apoptotic lymphocytes in blood is significantly higher compared with the unstressed animals. B-lymphocytes as well as Ig(-) lymphoid cells contributed to this increased apoptosis. Labelling of blood lymphocytes with a polyclonal antiserum against the glucocorticoid receptor also showed, besides B-lymphocytes, part of the Ig(-) lymphoid cell population to be glucocorticoid receptor positive. As the distribution of B-lymphocytes was substantially affected, the effect of temperature stress on T-lymphocyte-independent (trinitrophenyl-lipopolysaccharide) and T-lymphocyte-dependent (dinitrophenyl-keyhole limpet hemocyanin) humoral antibody responses was determined. Kinetics of the primary antibody response to the T-lymphocyte-independent antigen showed lower antibody titres in stressed carp during the onset of the immune response, implying a slower development of the antibody response against the T-lymphocyte-independent antigen. (C) 2003 Elsevier Ltd. All rights reserved.