Expression of the urokinase plasminogen activator receptor (uPAR) and its ligand (uPA) in brain tissues of human immunodeficiency virus patients with opportunistic cerebral diseases.
Expression of the urokinase plasminogen activator receptor (uPAR) and its ligand (uPA) in brain tissues of human immunodeficiency virus patients with opportunistic cerebral diseases.
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人类免疫缺陷病毒机会性脑病患者脑组织中尿激酶纤溶酶原激活剂受体（uPAR）及其配体（uPA）的表达。
DOI：
10.1080/13550280802400692
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发表时间：
2009
影响因子：
3.2
通讯作者：
Vago,Luca
中科院分区：
文献类型：
--
作者：
Nebuloni,Manuela;Cinque,Paola;Sidenius,Nicolai;Ferri,Angelita;Lauri,Eleonora;Omodeo-Zorini,Elisabetta;Zerbi,Pietro;Vago,Luca
尿激酶型纤溶酶原激活物受体（uPAR）及其配体（uPA）在细胞迁移和细胞外蛋白水解中起重要作用。我们以前描述了uPAR/uPA过度表达的脑脊液（CSF）和脑组织的患者与人类免疫缺陷病毒（HIV）相关的脑部疾病。在这项研究中，我们研究了uPAR/uPA的表达免疫组织化学（IHC）在大脑中的HIV患者的机会性脑病变和HIV阳性/阴性对照。uPAR在巨细胞病毒（CMV）脑炎、弓形体病和淋巴瘤中的巨噬细胞/小胶质细胞中的水平最高;在隐球菌病和进行性多灶性白质脑病（PML）病例中，仅发现少数阳性细胞，对照组中未观察到阳性。在所有病例和HIV阳性对照中，uPA仅在少数巨噬细胞/小胶质细胞和淋巴细胞中表达，没有不同的分布模式;在隐球菌病和HIV阴性对照中未发现uPA免疫染色。uPAR/uPA在大多数机会性脑损伤中的高表达支持它们在这些疾病中的作用，表明它们对组织损伤的贡献。
The urokinase plasminogen activator receptor (uPAR) and its ligand (uPA) play an important role in cell migration and extracellular proteolysis. We previously described uPAR/uPA overexpression in the cerebrospinal fluid (CSF) and brain tissues of patients with human immunodeficiency virus (HIV)-related cerebral diseases. In this study, we examined uPAR/uPA expression by immunohistochemistry (IHC) in brains of HIV patients with opportunistic cerebral lesions and in HIV-positive/negative controls. uPAR was found in macrophages/microglia with the highest levels in cytomegalovirus (CMV) encephalitis, toxoplasmosis, and lymphomas; in cryptococcosis and progressive multifocal leukoencephalopathy (PML) cases, only a few positive cells were found and no positivity was observed in controls. uPA expression was demonstrated only in a few macrophages/microglia and lymphocytes in all the cases and HIV-positive controls without different pattern of distribution; no uPA immunostaining was found in cryptococcosis and HIV-negative controls. The higher expression of uPAR/uPA in most of the opportunistic cerebral lesions supports their role in these diseases, suggesting their contribution to tissue injury.