BEHAVIORAL AND NEUROPATHOLOGICAL EFFECTS PRODUCED BY TETANUS TOXIN INJECTED INTO THE HIPPOCAMPUS OF RATS
BEHAVIORAL AND NEUROPATHOLOGICAL EFFECTS PRODUCED BY TETANUS TOXIN INJECTED INTO THE HIPPOCAMPUS OF RATS
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DOI：
10.1016/0028-3908(90)90130-j
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发表时间：
1990-08-01
影响因子：
4.7
通讯作者：
BOWERY, NG
中科院分区：
文献类型：
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作者：
BAGETTA, G;CORASANITI, MT;BOWERY, NG
在大鼠中研究了注射到嘴侧海马体的破伤风毒素的行为影响。单剂量（1000小鼠最小致死剂量; n = 10）的毒素产生尾部僵硬，驼背和声音和触摸诱发的刺激，48小时后，在所有大鼠治疗，这些最终在全身惊厥5-7天后。在注射2000 MLD（n = 10）后4天也观察到癫痫发作，而500 MLD（n = 10）的剂量无效。 同样，观察到剂量和时间依赖性致死效应。与对侧（未处理的）海马相比，破伤风毒素（1000 MLD; n = 3）在注射后7天和10天在注射侧的CA 1锥体细胞层中产生统计学显著的细胞数量减少。海马的其他部分（CA 2和CA 3区）未观察到变化。总之，目前的实验表明，局部注射破伤风毒素到海马产生剂量和时间依赖性的行为刺激和致死作用的大鼠。
The behavioural effects of tetanus toxin, injected into the rostral hippocampus, have been studied in rats. A single dose (1000 mouse minimum lethal doses; n = 10) of the toxin produced tail rigidity, hunched back and sound- and touch-evoked stimuli, 48 hr after the injection in all rats treated and these culminated in generalized convulsions 5-7 days later. Seizures were also observed 4 days after the injection of 2000 MLDs (n = 10), whereas a dose of 500 MLDs (n = 10) was ineffective. Similarly, dose- and time-dependent lethal effects was observed. In comparison to the contralateral (untreated) hippocampus, tetanus toxin (1000 MLDs; n = 3) produced a statistically significant reduction in the number of cells in the CA1 pyramidal cell layer of the injected side, 7 and 10 days after the injection. No changes were observed in other sectors (CA2 and CA3 areas) of the hippocampus. In conclusion, the present experiments have shown that the focal injection of tetanus toxin into the hippocampus produced dose- and time-dependent behavioural stimulation and lethal effects in rats.