Bisphenol S induced epigenetic and transcriptional changes in human breast cancer cell line MCF-7
Bisphenol S induced epigenetic and transcriptional changes in human breast cancer cell line MCF-7
复制标题
双酚 S 诱导人乳腺癌细胞系 MCF-7 的表观遗传和转录变化
DOI：
10.1016/j.envpol.2018.12.084
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发表时间：
2019-03-01
影响因子：
8.9
通讯作者：
Cai, Zongwei
中科院分区：
文献类型：
--
作者：
Huang, Wei;Zhao, Chao;Cai, Zongwei
近年来，人们对双酚A（BPA）在日常消费品中的使用及其对许多慢性人类疾病的影响的担忧促使BPA的去除。然而，广泛使用的BPA替代品，包括双酚S（BPS），与BPA具有高度的结构相似性，这表明它们可能对人类具有类似的生物效应。事实上，BPS也被发现具有内分泌干扰作用。在体外和体内模型中，BPA诱导的生物学效应均涉及表观遗传机制。然而，没有评估BPS是否会导致表观遗传变化。在这项工作中，我们研究了BPS可能诱导人乳腺癌细胞系MCF-7的表观遗传效应。我们发现BPS可以改变转座子的DNA甲基化水平。此外，乳腺癌相关基因CDH 1、SFN、TNFRSF 10 C启动子区甲基化状态也发生了改变，提示BPS可能在乳腺癌的发生发展中起作用。基因表达谱分析显示，一些与乳腺癌进展相关的基因表达上调，包括THBS 4、PPARGC 1A、CREB 5、COL 5A 3。基因本体（GO）分析显示，差异表达基因中PI 3 K-Akt信号通路和细胞外基质发生了显著变化，与乳腺癌细胞的增殖、迁移和侵袭有关。这些结果表明，BPS暴露可能在乳腺癌的进展中发挥作用。(C)2019爱思唯尔有限公司版权所有。
In recent years, concerns about using Bisphenol A (BPA) in daily consume products and its effects in many chronic human diseases have prompted the removal of BPA. However, the widely used BPA alternatives, including Bisphenol S (BPS), have a high structural similarity with BPA, suggesting that they may have similar biological effects towards human beings. Indeed, BPS was also found to have endocrine disrupting effects. Epigenetic mechanism was reported to be involved in BPA-induced biological effects in both in vitro and in vivo models. However, there is no assessment on whether BPS could cause epigenetic changes. In this work, we investigated the possible epigenetic effects of BPS that might induce in human breast cancer cell line MCF-7. We found that BPS could change DNA methylation level of transposons. Besides, methylation status in promoter of breast cancer related genes CDH1, SFN, TNFRSF10C were also changed, which implied that BPS might play a role in the development of breast cancer. Gene expression profiling showed that some genes related to breast cancer progression were upregulated, including THBS4, PPARGC1A, CREB5, COL5A3. Gene ontology (GO) analysis of the differentially expressed genes revealed the significantly changes in PI3K-Akt signaling pathway and extracellular matrix, which were related to the proliferation, migration and invasion of breast cancer cells. These results illustrated that BPS exposure might play roles in the progression of breast cancer. (C) 2019 Elsevier Ltd. All rights reserved.