The complete DNA sequence of the Ectocarpus siliculosus virus EsV-1 genome
The complete DNA sequence of the Ectocarpus siliculosus virus EsV-1 genome
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DOI：
10.1006/viro.2001.1028
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发表时间：
2001-08-15
期刊：
影响因子：
3.7
通讯作者：
Boland, W
中科院分区：
文献类型：
--
作者：
Delaroque, N;Müller, DG;Boland, W
硅壳外果皮病毒1（Ectocarpus siliculosus Virus-1，EsV-1）是藻DNA病毒科（Phaeoviruses）的模式种，感染海洋丝状褐藻。EsV-1基因组335，593 bp，除了大量开放阅读框外，还含有串联和分散的重复元件，其中231个开放阅读框目前被计为基因。许多基因可以被分配到参与DNA合成、DNA整合、转座和多糖代谢的功能组。此外，EsV-1含有一个令人惊讶的复杂的信号转导系统与六个不同的杂合组氨酸蛋白激酶和四个推定的丝氨酸/苏氨酸蛋白激酶的组件。其他几个基因编码具有蛋白质-蛋白质相互作用结构域的多肽。然而，50%的预测基因在数据库中没有对应物。在231个已鉴定的基因中，只有28个与另一种藻DNA病毒小球藻病毒PBCV-1的基因具有显著的序列相似性。据我们所知，EsV-1基因组是迄今为止最大的病毒DIVA测序。(C)北京：科学出版社.
The Ectocarpus siliculosus Virus-1, EsV-1, is the type-species of a genus of Phycodnaviridae, the phaeoviruses, infecting marine filamentous brown algae. The EsV-1 genome of 335,593 by contains tandem and dispersed repetitive elements in addition to a large number of open reading frames of which 231 are currently counted as genes. Many genes can be assigned to functional groups involved in DNA synthesis, DNA integration, transposition, and polysaccharide metabolism. Furthermore, EsV-1 contains components of a surprisingly complex signal transduction system with six different hybrid histidine protein kinases and four putative serine/threonine protein kinases. Several other genes encode polypeptides with protein-protein interaction domains. However, 50% of the predicted genes have no counterparts in data banks. Only 28 of the 231 identified genes have significant sequence similarities to genes of the Chlorella virus PBCV-1, another phycodnavirus. To our knowledge, the EsV-1 genome is the largest viral DIVA sequenced to date. (C) 2001 Academic Press.