Electrical impedance spectroscopy of single cells in hydrodynamic traps
Electrical impedance spectroscopy of single cells in hydrodynamic traps
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DOI：
10.1016/j.snb.2017.04.019
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发表时间：
2017-09-01
影响因子：
8.4
通讯作者：
Schnakenberg, Uwe
中科院分区：
文献类型：
--
作者：
El Hasni, Akram;Schmitz, Carlo;Schnakenberg, Uwe
本文提出了一种新的设计，结合流体动力学捕获和阻抗谱测量的单细胞的微流控装置。集成在细胞陷阱内的四个微电极可以在不同的电极对配置中进行阻抗测量。为了改善微电极的阻抗响应，施加使用导电聚合物的电沉积的改性。观察到阻抗幅值的相当大的减小，并且因此获得了有用频率范围的显著增强。电极改性后，12 × 22 μ m（2）电极对10 KHz至2-5 MHz的频率范围敏感。对具有和不具有周围糖蛋白基质（称为透明带）的单个小鼠卵母细胞进行了阻抗测量。更高的阻抗值，获得了透明质酸的自由比透明质酸完整的卵母细胞，反映了已知的高电导率的透明质酸。(C)2017爱思唯尔B. V.保留所有权利。
This paper presents a new design of a microfluidic device combining hydrodynamic trapping and impedance spectroscopy measurements of single cells. Four microelectrodes integrated within cell traps enable impedance measurements in varying electrode pair configurations. To improve the impedance response of the microelectrodes, a modification using electrodeposition of a conductive polymer was applied. A considerable decrease of impedance magnitude was observed, and thus a significant enhancement of the useful frequency range was obtained. After electrode modification, the 12 x 22 mu m(2) electrodes were sensitive for frequencies ranging from 10 KHz to 2-5 MHz. Impedance measurements were carried out on single mouse oocytes with and without the surrounding glycoprotein matrix, called zona pellucida. Higher impedance values were obtained for zona pellucida-free than for zona pellucida-intact oocytes, reflecting the known high electrical conductivity of the zona pellucida. (C) 2017 Elsevier B.V. All rights reserved.