Biophysical and biochemical characterization of hepatitis A virus.
Biophysical and biochemical characterization of hepatitis A virus.
复制标题
甲型肝炎病毒的生物物理和生化特征。
作者：
A. Coulepis;S. Locarnini;E. G. Westaway;G. Tannock;I. Gust
甲型肝炎病毒的生物物理和生物化学分析表明，它是一个27至32纳米的二十面体颗粒，有32个壳粒。成熟病毒粒子的浮力密度为1.33-1.34 g/cm 3，沉降系数为156- 160 S，由分子量为30，000 - 33，000（VP 1）、24，000 - 27，000（VP 2）、21，000 - 23，000（VP 3）和7，000 - 14，000（VP 4）的四种多肽组成。甲型肝炎病毒的基因组由一段单链RNA组成，其在32- 35 S处沉积，浮力密度为1.64 g/cm 3。在非变性条件下测量时，RNA的分子量为2.25 × 10（6），在完全变性条件下测量时为2.8 × 10（6）。基因组含有40-80个核苷酸的多聚腺苷酸序列，能够感染细胞培养物。这些发现，以及观察到病毒粒子在pH 3.0下稳定，并且对乙醚和60度温度下1小时具有抗性，表明甲型肝炎病毒现在应被归类为小核糖核酸病毒科中的肠道病毒。
Biophysical and biochemical analysis of hepatitis A virus has shown it to be a 27- to 32-nm icosahedral particle with 32 capsomers. The mature virion has a buoyant density of 1.33-1.34 g/cm3, a sedimentation coefficient of 156-160S, and is composed of four polypeptides with molecular weights of 30,000-33,000 (VP1), 24,000-27,000 (VP2), 21,000-23,000 (VP3), and 7,000-14,000 (VP4). The genome of hepatitis A virus consists of a single piece of single-stranded RNA which sediments at 32-35S and has a buoyant density of 1.64 g/cm3. The molecular weight of RNA is 2.25 x 10(6) when measured under nondenaturing conditions and 2.8 x 10(6) when measured under fully denaturing conditions. The genome contains a 40-80 nucleotide sequence of polyadenylic and is capable of infecting cell cultures. These findings, together with the observation that the virion is stable at pH 3.0 and resistant to ether and a temperature of 60 degrees for 1 h, indicate that hepatitis A virus should now be classified as an Enterovirus within the family Picornaviridae.