Epicutaneous immunization with hapten-conjugated protein antigen alleviates contact sensitivity mediated by three different types of effector cells
Epicutaneous immunization with hapten-conjugated protein antigen alleviates contact sensitivity mediated by three different types of effector cells
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DOI：
10.1016/s1734-1140(12)70887-3
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发表时间：
2012-07-01
影响因子：
4.4
通讯作者：
Szczepanik, Marian
中科院分区：
文献类型：
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作者：
Majewska-Szczepanik, Monika;Strzepa, Anna;Szczepanik, Marian
背景：变态反应性接触性皮炎（ACD）是工业化国家常见的临床疾病，常引起职业病。接触敏感动物模型（CS）是研究小鼠ACD的常用方法，可通过半抗原皮肤应用诱导。先前已经证明CS是由CD4（+）或CD8(+) T效应细胞介导的。最近又发现肝NK细胞在小鼠体内也能发挥CS效应细胞的作用。方法：本研究的目的是测试皮肤诱导的抑制是否可以抑制体内的CS反应。结果：本研究表明，在半抗原致敏之前，用半抗原结合蛋白抗原免疫正常小鼠EC可抑制Th1、Tc1和NK介导的CS反应。结论：这些数据强烈表明，EC免疫的操作可能对设计旨在调节接触性超敏反应的不良免疫反应的治疗方案具有重要意义。
Background: Allergic contact dermatitis (ACD) is a common clinical condition in industrialized countries and often causes occupational diseases. Animal model of contact sensitivity (CS) is commonly used to study ACD in mice and can be induced by skin application of haptens. It has been previously shown that CS is mediated by CD4(+) or CD8(+) T effector cells. More recently it was found that also liver NK cells can play a role of CS effector cells in mice.Methods: The aim of the present study was to test whether skin-induced suppression could inhibit CS response in vivo.Results: Here we show that EC immunization of normal mice with hapten conjugated protein antigen prior to hapten sensitization suppresses Th1, Tc1 and NK mediated CS responses.Conclusions: These data strongly suggest that maneuver of EC immunization may have important implications for designing therapeutic schemes aimed at modulating unwanted immune responses in contact hypersensitivity.