Mirror-Image 5S Ribonucleoprotein Complexes
Mirror-Image 5S Ribonucleoprotein Complexes
复制标题
镜像 5S 核糖核蛋白复合物
DOI：
10.1002/anie.201914799
复制
发表时间：
2020-01-21
影响因子：
16.6
通讯作者：
Zhu, Ting F.
中科院分区：
文献类型：
--
作者：
Ling, Jun-Jie;Fan, Chuyao;Zhu, Ting F.
在实现镜像基因复制、转录和逆转录之后，建立镜像版本的中心法则的最大挑战是建立基于镜像核糖体的翻译机器。在这里，我们化学合成的自然和镜像版本的三个核糖体蛋白（L5，L18和L25）的大肠杆菌核糖体大亚基的翻译后修饰。我们表明，合成的镜像蛋白可以在体外折叠，尽管效率有限，并与酶促转录的镜像5S核糖体RNA组装成核糖核蛋白复合物。此外，RNA-蛋白质相互作用是手性特异性的，因为镜像核糖体蛋白不与天然5S核糖体RNA结合，反之亦然。镜像5S核糖核蛋白复合物的合成和组装是构建功能性镜像核糖体的重要步骤。
After realizing mirror-image genetic replication, transcription, and reverse transcription, the biggest challenge in establishing a mirror-image version of the central dogma is to build a mirror-image ribosome-based translation machine. Here, we chemically synthesized the natural and mirror-image versions of three ribosomal proteins (L5, L18, and L25) in the large subunit of the Escherichia coli ribosome with post-translational modifications. We show that the synthetic mirror-image proteins can fold in vitro despite limited efficiency and assemble with enzymatically transcribed mirror-image 5S ribosomal RNA into ribonucleoprotein complexes. In addition, the RNA-protein interactions are chiral-specific in that the mirror-image ribosomal proteins do not bind with natural 5S ribosomal RNA and vice versa. The synthesis and assembly of mirror-image 5S ribonucleoprotein complexes are important steps towards building a functional mirror-image ribosome.