Polysaccharide of “Junsai (Brasenia schreberi J. F. Gmel)” Mucilage: Constitution and Linkage Analysis
Polysaccharide of “Junsai (Brasenia schreberi J. F. Gmel)” Mucilage: Constitution and Linkage Analysis
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“莼菜（Brasenia schreberi J. F. Gmel）”粘液多糖：组成和连锁分析
DOI：
10.1271/bbb1961.43.993
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
A. Misaki
中科院分区：
文献类型：
--
作者：
M. Kakuta;A. Misaki
用热水提取和氯化十六烷基吡啶分离纯化了菌塞(Brasenia schreberi J.F.Gmel)幼叶和茎中的酸性多糖。多糖由D-半乳糖(32~40%)、D-甘露糖(10~14%)、L岩藻糖(13~16%)、L鼠李糖(6~9%)、D-木糖(2~7%)、L-阿拉伯糖(2~3%)、D-葡萄糖醛酸(19~29%)和少量D-葡萄糖组成。这些比例是不同的，取决于栖息地。甲基化多糖经酸水解得到17个以上的甲基糖片段，包括2，4，6-三和3，4，6-三-和3，4-O-甲基-D-半乳糖、2，3-二-O-甲基-D-葡萄糖醛酸、2，4-二-O-甲基-L-岩藻糖、2，4-二-O-甲基-L-鼠李糖、二和单-O-甲基-D-半乳糖和-D-甘露糖，以及三-O-甲基-L-阿拉伯糖、-d-木糖、-L-岩藻糖、-L-鼠李糖和四-O-甲基-D-半乳糖。这些结果表明，该多糖具有高度的分枝结构，主要由(L→3)连接的D-半乳糖、L-鼠李糖和L-岩藻糖(L→2)连接.
An acidic polysaccharide which coats young leaves and stems of Junsai (Brasenia schreberi J. F. Gmel) was extracted with hot water and purified with cetylpyridinium chloride. The polysaccharide comprises d-galactose (32~40%), d-mannose (10~14%), l-fucose (13~16%), l-rhamnose (6~9%), d-xylose (2~7%), l-arabinose (2~3%), d-glucuronic acid (19~29%) and a trace of d-glucose. These proportions are different, depending on the habitats. Methylated polysaccharide yielded, on acid hydrolysis, more than 17 methyl sugar fragments, which included 2, 4, 6-tri- and 3, 4, 6-tri-O-methyl-d-galactose, 2, 3-di-O-methyl-d-glucuronic acid, 2,4-di-O-methyl-l-fucose, 2,4-di-O-methyl-l-rhamnose, di- and mono-O-methyl-d-galactoses and -d-mannoses, in addition to tri-O-methyl-l-arabinose, -d-xylose, -l-fucose, -l-rhamnose and tetra-O-methyl-d-galactose. These results indicate that the polysaccharide has an extremely highly branched structure, consisting mainly of (l → 3)-linked d-galactose, l-rhamnose and l-fucose, (l → 2)-linked...