CHARACTERIZATION OF DNA FROM THE DINOFLAGELLATE WOLOSZYNSKIA-BOSTONIENSIS
CHARACTERIZATION OF DNA FROM THE DINOFLAGELLATE WOLOSZYNSKIA-BOSTONIENSIS
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DOI：
10.1111/j.1550-7408.1988.tb04120.x
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发表时间：
1988-08-01
期刊：
影响因子：
--
通讯作者：
NORDBY, O
中科院分区：
文献类型：
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作者：
DAVIES, W;JAKOBSEN, KS;NORDBY, O
Some physical and chemical properties of DNA isolated from the dinoflagellate Woloszynskia bostoniensis were determined. Analytical cesium chloride gradient centrifugation gave a major component and a minor component banding at 1.719 and 1.693 g.cm, respectively. Thermal denaturation in 0.1 SSC showed a broad transition with a Tm of 70.5.degree. C. Derivation of this curve indicated that two components were present having Tm values of 66.degree. C and 70.degree. C. Base composition analysis showed a GC content of 48.1% and a high degree of thymine replacement by 5-hydroxymethyluracil. Two minor bases, identified as 5-methylcytosine and N6-methyladenine, were also detected. Reassociation kinetics showed a typical eukaryotic reassociation pattern with 45% repetitive and 55% single copy sequences.
