Water intake increases mesenteric lymph flow and the total flux of albumin, long-chain fatty acids, and IL-22 in rats: new concept of absorption in jejunum
Water intake increases mesenteric lymph flow and the total flux of albumin, long-chain fatty acids, and IL-22 in rats: new concept of absorption in jejunum
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DOI：
10.1152/ajpgi.00325.2018
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发表时间：
2019-01-01
影响因子：
4.5
通讯作者：
Ohhashi, Tashio
中科院分区：
文献类型：
--
作者：
Nagashio, Sachiho;Ajima, Kumiko;Ohhashi, Tashio
日本传统的保健习俗建议人们摄入适量的水。然而，支持这一做法的生理和免疫学机制尚不清楚。因此，我们通过大鼠和兔的活体实验，研究了蒸馏水灌胃对空肠起源的淋巴流量以及肠系膜淋巴管中细胞、白蛋白、长链脂肪酸和天然淋巴样细胞3(ILC-3)分泌的白介素22(IL-22)浓度和总流量的影响。用流式细胞仪和RT-PCR检测ILC-3在饮水大鼠小肠中的分布和活性。灌胃蒸馏水使大鼠肠系膜淋巴流量显著增加，淋巴管细胞、白蛋白、长链脂肪酸和IL-22的总流量显著增加。静脉注射伊文思蓝后，伊文思蓝迅速进入兔肠系膜淋巴管和乳糜池。ILC-3在大鼠空肠固有层细胞中分布最广，IL-22mRNA表达最强。在对照组和饮水条件下，没有观察到ILC-3在淋巴中的显著存在。总之，空肠吸收的水分是通过肠系膜淋巴管运输的。白蛋白在空肠微循环中的较高通透性可能在消耗的水的运输以及长链脂肪酸的储存和运输中发挥关键作用。水的摄入也加速了IL-22向肠系膜淋巴的转移，这可能在一定程度上有助于维持和促进体内的先天免疫。值得注意的是，空肠系膜淋巴管中由白蛋白介导的水溶性物质的较高渗透性可能对经典的概念产生了很大的影响，表明水溶性小分子通过门静脉进入肝脏。ILC-3主要位于空肠固有层，尤其是其上部。从ILC-3释放的IL-22也通过肠系膜淋巴与白蛋白介导的消耗的水的运动协同运输。
The traditional Japanese health care custom recommends that a suitable volume of water is consumed. However, physiological and immunological mechanisms in support of this practice are unknown. Therefore, we conducted rat and rabbit in vivo experiments to investigate the effects of intragastric administration of distilled water on the jejunal-originated lymph flow and the concentrations and total flux of cells, albumin, long-chain fatty acids, and innate lymphoid cell 3 (ILC-3)-secreted interleukin-22 (IL-22) through mesenteric lymph vessels. The distribution and activity of ILC-3 in rat small intestine by water intake were evaluated using flow cytometry and RT-PCR. The intragastric administration of distilled water caused significant increases in rat mesenteric lymph flow and in the total flux of cells, albumin, long-chain fatty acids, and IL-22 through the lymph vessels. Intravenously injected Evans blue dye was rapidly transported into rabbit mesenteric lymph vessel and cisterna chyli. The distribution of ILC-3 and the expression of IL-22 mRNA were maximal in the lamina propria cells of the rat jejunum. No significant presence of ILC-3 in the lymph was observed in the control and under water intake conditions. In conclusion, the absorbed water in the jejunum is transported through mesenteric lymph vessels. The higher permeability of albumin in the jejunal microcirculation may play key roles in the transport of consumed water and the reservoir and transporter of long-chain fatty acids. Water intake also accelerates the transfer of IL-22 to the mesenteric lymph, which may contribute, in part, to maintaining and promoting the innate immunity in the body.NEW & NOTEWORTHY The higher permeability of album-inmediated transport of water-soluble substances in mesenteric lymph vessels of the jejunum may have a large impact on the classic concept suggesting that water-soluble small molecules travel to the liver via the portal vein. ILC-3 is mainly housed in the lamina propria of the jejunum, especially its upper part. IL-22 released from the ILC-3 is also transported through mesenteric lymph in collaboration with the albumin-mediated movement of consumed water.