Vasoactive peptides and phenylephrine actions in isolated teleost hepatocytes.
Vasoactive peptides and phenylephrine actions in isolated teleost hepatocytes.
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血管活性肽和去氧肾上腺素在分离的硬骨鱼肝细胞中的作用。
DOI：
10.1152/ajpendo.1990.259.5.e644
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
T. Mommsen
中科院分区：
文献类型：
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作者：
T. W. Moon;T. Mommsen
本文研究了从三种硬骨鱼（褐头鱼、虹鳟和美洲鳗鱼）中分离的肝细胞中血管活性肽缩后素、异后素和α激动剂苯肾上腺素的代谢作用。这三种化合物对肝脏糖异生和糖原分解有显著的物种差异。血管催产素和异源催产素对鳗鱼肝细胞激活糖异生的影响仅为1.7倍，对糖原溶解的影响为3倍。苯肾上腺素使牛头肝细胞的糖原溶解增加7倍，使鳟鱼细胞的糖异生增加1.4倍。催产素和苯肾上腺素的作用与腺苷3′，5′-环单磷酸腺苷（cAMP）升高相关。血管催产素的作用不受β和α拮抗剂支持鳗鱼肝细胞上的v2型受体的影响。普萘洛尔消除了苯肾上腺素的作用，吡唑嗪和育亨宾（分别为α 1-和α 2-拮抗剂）则降低了苯肾上腺素的作用。因此，苯肾上腺素通过强调-肾上腺素受体的混合α / β受体机制或为哺乳动物定义的经典α / β激动剂/拮抗剂方案影响鱼肝细胞代谢，不适用于这些鱼类制剂。
The metabolic actions of the vasoactive peptides vasotocin and isotocin and the alpha-agonist phenylephrine are examined in hepatocytes isolated from three teleost species: brown bullhead, rainbow trout, and American eel. These three compounds influenced hepatic gluconeogenesis and glycogenolysis with significant species differences. Vasotocin and isotocin affected only eel hepatocytes activating gluconeogenesis by 1.7-fold and glycogenolysis by 3-fold. Phenylephrine increased glycogenolysis by 7-fold in bullhead hepatocytes and gluconeogenesis by 1.4-fold in trout cells. Vasotocin and phenylephrine actions were correlated with increases in adenosine 3',5'-cyclic monophosphate (cAMP). The vasotocin effects were unaffected by beta- and alpha-antagonists supporting a V2-type receptor on eel hepatocytes. Phenylephrine effects were abolished by propranolol and reduced by prazosin and yohimbine (alpha 1- and alpha 2-antagonists, respectively). Phenylephrine, therefore, affected fish hepatocyte metabolism either by a mixed alpha/beta-receptor mechanism emphasizing beta-adrenoceptors or the classic alpha/beta agonist/antagonist scheme defined for mammals is not appropriate for these fish preparations.