Fixation, embedding, and sectioning of tissues, embryos, and single cells.
Fixation, embedding, and sectioning of tissues, embryos, and single cells.
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DOI：
10.1002/0471141755.pha03ds07
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发表时间：
1999-12
影响因子：
--
通讯作者：
R. Zeller
中科院分区：
文献类型：
--
作者：
R. Zeller
本单元描述了固定和切片各种形式的生物材料，从组织到单细胞的选择方法。根据这些方案制备的切片可用于检查细胞和组织形态，并用于原位杂交、免疫组织化学和酶组织化学研究。基本方案描述了如何通过在多聚甲醛中固定然后在蜡中包埋来制备组织和胚胎以进行切片，而替代方案描述了悬浮或培养细胞的固定。两个支持协议包括保存和固定器官灌注整个动物与多聚甲醛和切片蜡块固定组织的程序，加上随后安装到准备好的或“subbed”的玻璃载玻片。
This unit describes selected methods for fixing and sectioning various forms of biological materials ranging from tissues to single cells. Sections prepared according to these protocols can then be used to examine cell and tissue morphology and in studies involving in situ hybridization, immunohistochemistry, and enzyme histochemistry. The basic protocol describes how tissues and embryos can be prepared for sectioning by fixing in paraformaldehyde followed by embedding in wax, while an alternate protocol describes fixation of suspended or cultured cells. Two support protocols cover preserving and fixing organs by perfusion of whole animals with paraformaldehyde and a procedure for sectioning wax blocks of fixed tissue, plus the subsequent mounting of sections onto prepared or "subbed" glass slides.