Gene expression of adiponectin receptors in human visceral and subcutaneous adipose tissue is related to insulin resistance and metabolic parameters and is altered in response to physical training
Gene expression of adiponectin receptors in human visceral and subcutaneous adipose tissue is related to insulin resistance and metabolic parameters and is altered in response to physical training
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DOI：
10.2337/dc07-1257
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发表时间：
2007-12-01
期刊：
影响因子：
16.2
通讯作者：
Mantzoros, Christos S.
中科院分区：
文献类型：
--
作者：
Blueher, Matthias;Williams, Catherine J.;Mantzoros, Christos S.
目的-脂联素受体I和2(分别为AdipoR1和AdipoR2)介导脂联素对体内糖脂代谢的影响。我们研究了人类脂肪组织中AdipoR1和/或AdipoR2的mRNA表达是否具有脂肪库特异性。研究设计与方法：在153名受试者的观察性研究和60名受试者(糖耐量正常、糖耐量受损和2型糖尿病患者各20名)的干预研究中，我们测定了代谢参数，并用实时定量聚合酶链式反应技术评价了脂肪组织中AdipoR1和AdipoR2的mRNA表达。AdipoR2在内脏脂肪中的表达与其在皮下脂肪中的表达高度相关。内脏脂肪和皮下脂肪中AdipoR2 mRNA的表达与循环脂联素和高密度脂蛋白水平呈正相关，而与肥胖以及调整脂肪质量前后的胰岛素抵抗、血糖和其他血脂水平呈负相关。运动4周后，皮下脂肪中AdipoR1和AdipoR2的mRNA表达增加。结论脂肪中AdipoR2的表达与胰岛素抵抗和代谢参数呈负相关，与肥胖无关，并可能介导运动后胰岛素抵抗的改善。
OBJECTIVE - Adiponectin receptors I and 2 (AdipoR1 and AdipoR2, respectively) mediate the effects of adiponectin on glucose and lipid Metabolism in vivo. We examined whether AdipoR1 and/or AdipoR2 mRNA expression in human adipose tissue is fat-depot specific. We also studied whether their expression in visceral and subcutaneous fat depots is associated with metabolic parameters and whether their expression is regulated by intensive physical exercise.RESEARCH DESIGN AND METHODS - We determined metabolic parameters and assessed AdipoR1 and AdipoR2 mRNA expression using quantitative real-time PCR in adipose tissue in an observational study of 153 subjects and an interventional study of 60 subjects (20 each with normal glucose tolerance, impaired glucose tolerance, and type 2 diabetes) before and after intensive physical training for 4 weeks.RESULTS - AdipoR1 and AdipoR2 mRNA expression is not significantly different between omental and subcutaneous fat, but their expression is several-fold lower in adipose tissue than in muscle. AdipoR2 mRNA expression in visceral fat is highly correlated with its expression in subcutaneous fat. AdipoR2 mRNA expression in both visceral and subcutaneous fat is positively associated with circulating adiponectin and HDL levels but negatively associated with obesity as well as parameters of insulin resistance, glycemia, and other lipid levels before and after adjustment for fat mass. Physical training for 4 weeks resulted in increased AdipoR1 and AdipoR2 mRNA expression in subcutaneous fat.CONCLUSIONS - AdipoR2 mRNA expression in fat is negatively associated with insulin resistance and metabolic parameters independently of obesity and may mediate the improvement of insulin resistance in response to exercise.