Soy Glycinin Contains a Functional Inhibitory Sequence against Muscle-Atrophy-Associated Ubiquitin Ligase Cbl-b.
Soy Glycinin Contains a Functional Inhibitory Sequence against Muscle-Atrophy-Associated Ubiquitin Ligase Cbl-b.
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DOI：
10.1155/2013/907565
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发表时间：
2013
影响因子：
2.8
通讯作者：
Nikawa T
中科院分区：
文献类型：
--
作者：
Abe T;Kohno S;Yama T;Ochi A;Suto T;Hirasaka K;Ohno A;Teshima-Kondo S;Okumura Y;Oarada M;Choi I;Mukai R;Terao J;Nikawa T
背景去负荷应激诱发骨骼肌萎缩。我们已经报道，Cbl-b泛素连接酶是一个主调节卸载相关的肌肉萎缩。本研究的目的是阐明是否饮食大豆球蛋白预防失神经介导的肌肉萎缩，基于对大豆球蛋白中的Cbl-b泛素连接酶的抑制肽的存在。方法.小鼠喂食20%酪蛋白饮食、20%大豆蛋白分离物饮食、含有10%酪蛋白的10%大豆球蛋白饮食或20%大豆球蛋白饮食。一周后，右侧坐骨神经被切断。在失神经支配后1、3、3.5或4天检测后肢肌肉的湿重、横截面积（CSA）、IGF-1信号和atrogene表达。结果20%大豆球蛋白饮食可显着防止去神经诱导的肌肉湿重和肌纤维CSA下降。此外，膳食大豆蛋白抑制去神经诱导的胫骨前肌IRS-1的泛素化和降解。膳食大豆球蛋白部分抑制失神经介导的atrogenes，如MAFbx/atrogin-1和MuRF-1的表达，通过保护IGF-1信号估计Akt-1的磷酸化。结论.大豆球蛋白含有对肌肉萎缩相关的泛素连接酶Cbl-b的功能性抑制序列。饮食大豆球蛋白显着防止肌肉萎缩后，小鼠去神经支配。
Background. Unloading stress induces skeletal muscle atrophy. We have reported that Cbl-b ubiquitin ligase is a master regulator of unloading-associated muscle atrophy. The present study was designed to elucidate whether dietary soy glycinin protein prevents denervation-mediated muscle atrophy, based on the presence of inhibitory peptides against Cbl-b ubiquitin ligase in soy glycinin protein. Methods. Mice were fed either 20% casein diet, 20% soy protein isolate diet, 10% glycinin diet containing 10% casein, or 20% glycinin diet. One week later, the right sciatic nerve was cut. The wet weight, cross sectional area (CSA), IGF-1 signaling, and atrogene expression in hindlimb muscles were examined at 1, 3, 3.5, or 4 days after denervation. Results. 20% soy glycinin diet significantly prevented denervation-induced decreases in muscle wet weight and myofiber CSA. Furthermore, dietary soy protein inhibited denervation-induced ubiquitination and degradation of IRS-1 in tibialis anterior muscle. Dietary soy glycinin partially suppressed the denervation-mediated expression of atrogenes, such as MAFbx/atrogin-1 and MuRF-1, through the protection of IGF-1 signaling estimated by phosphorylation of Akt-1. Conclusions. Soy glycinin contains a functional inhibitory sequence against muscle-atrophy-associated ubiquitin ligase Cbl-b. Dietary soy glycinin protein significantly prevented muscle atrophy after denervation in mice.
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DOI：
10.1016/j.bbamcr.2004.09.013
发表时间：
2005-03-22
影响因子：
5.1
作者：
Hirasaka, K;Nikawa, T;Kishi, K
通讯作者：
Kishi, K
影响因子：
4.4
作者：
Nikawa, T;Ikemoto, M;Kishi, K
通讯作者：
Kishi, K
影响因子：
3.4
作者：
Ogawa, Takayuki;Furochi, Harumi;Nikawa, Takeshi
通讯作者：
Nikawa, Takeshi
影响因子：
4.2
作者：
Anthony, Tracy G.;McDaniel, Brent J.;McNurlan, Margaret A.
通讯作者：
McNurlan, Margaret A.
影响因子：
3.4
作者：
Kohno, Shohei;Ueji, Tatsuya;Nikawa, Takeshi
通讯作者：
Nikawa, Takeshi