IDENTIFICATION AND ISOLATION OF YEAST CYTOCHROME-C GENE
IDENTIFICATION AND ISOLATION OF YEAST CYTOCHROME-C GENE
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DOI：
10.1016/0092-8674(78)90250-7
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发表时间：
1978-01-01
期刊：
影响因子：
64.5
通讯作者：
SMITH, M
中科院分区：
文献类型：
--
作者：
MONTGOMERY, DL;HALL, BD;SMITH, M
利用人工合成的寡脱氧核苷酸作为杂交探针，对酵母的iso1 -细胞色素c基因进行了鉴定和克隆。低聚物d[pT-T-A-G-C-A-G-A-A-C-C-G-G]与酵母cyc1基因N端编码区附近的一个区域互补。在与该探针杂交的几个酵母Eco RI片段中，一个片段的大小发生了G .fwdarw的变化。T突变消除了cyc1基因内的Eco RI位点。野生型和RI-突变型均在.lambda中克隆。gt向量。在iso-1-细胞色素c编码区对91个核苷酸进行测序，得到了与已知蛋白序列完全对应的DNA序列。
The iso-1-cytochrome c gene of yeast was identified and cloned using a synthetic oligodeoxynucleotide as a hybridization probe. The oligomer d[pT-T-A-G-C-A-G-A-A-C-C-G-G] is complementary to a region near the N terminal coding region of the yeast cyc1 gene. Of several yeast Eco RI fragments which hybridize to this probe, one is changed in size by a G .fwdarw. T mutation which eliminates an Eco RI site within the cyc1 gene. Both the wild-type and the RI- mutant forms were cloned in .lambda. gt vectors. Maxam-Gilbert sequencing for 91 nucleotides into the coding region for iso-1-cytochrome c yielded a DNA sequence in perfect correspondence with the known protein sequence.