Determination of cell division axes in the early embryogenesis of Caenorhabditis elegans.
Determination of cell division axes in the early embryogenesis of Caenorhabditis elegans.
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DOI：
10.1083/jcb.105.5.2123
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发表时间：
1987-11
期刊：
影响因子：
--
通讯作者：
White JG
中科院分区：
文献类型：
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作者：
Hyman AA;White JG
研究了秀丽隐杆线虫早期胚胎分裂时细胞分裂轴的建立。以前已经表明，在胚胎发生早期有两种不同的卵裂模式。在以决定性分裂为主的一组细胞中，分裂轴沿同一方向依次建立，而在以增殖分裂为主的另一组细胞中，分裂轴呈正交分裂模式。我们通过跟踪中心体的运动来研究这些轴的建立。中心体的分离在所有细胞中都遵循一种可复制的模式，这种模式本身导致了一个正交的分裂模式。在那些沿同一轴分裂的细胞中，中心体对与细胞核一起以明确的角度进行定向旋转。旋转需要完整的微管；微管的聚合和解聚抑制剂阻止了旋转。我们研究了固定胚胎在旋转过程中微管的分布。根据这些和其他数据，我们推断从中心体到皮层的微管在中心体-核复合体的排列中起着核心作用。
The establishment of cell division axes was examined in the early embryonic divisions of Caenorhabditis elegans. It has been shown previously that there are two different patterns of cleavage during early embryogenesis. In one set of cells, which undergo predominantly determinative divisions, the division axes are established successively in the same orientation, while division axes in the other set, which divide mainly proliferatively, have an orthogonal pattern of division. We have investigated the establishment of these axes by following the movement of the centrosomes. Centrosome separation follows a reproducible pattern in all cells, and this pattern by itself results in an orthogonal pattern of cleavage. In those cells that divide on the same axis, there is an additional directed rotation of pairs of centrosomes together with the nucleus through well-defined angles. Intact microtubules are required for rotation; rotation is prevented by inhibitors of polymerization and depolymerization of microtubules. We have examined the distribution of microtubules in fixed embryos during rotation. From these and other data we infer that microtubules running from the centrosome to the cortex have a central role in aligning the centrosome-nuclear complex.