Ultraviolet-B sensitivities in Japanese lowland rice cultivars: cyclobutane pyrimidine dimer photolyase activity and gene mutation.
Ultraviolet-B sensitivities in Japanese lowland rice cultivars: cyclobutane pyrimidine dimer photolyase activity and gene mutation.
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DOI：
10.1093/pcp/pch215
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发表时间：
2004-12
影响因子：
4.9
通讯作者：
Mika Teranishi;Y. Iwamatsu;J. Hidema;T. Kumagai
中科院分区：
文献类型：
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作者：
Mika Teranishi;Y. Iwamatsu;J. Hidema;T. Kumagai
水稻（Oryza sativa L.）对紫外线b （UVB: 280 ~ 320 nm）的响应存在品种差异。在日本的低地水稻品种中，日本的主要品种Sasanishiki对UVB的破坏作用具有抗性，而其近亲Norin 1的抗性较弱。我们发现Norin 1缺乏环丁烷嘧啶二聚体（cyclobutane嘧啶dimer， CPD）光修复能力，这表明水稻对UVB的敏感性很大程度上取决于CPD光修复能力。为了验证这一结论，我们在抗紫外线的Sasanishiki和抗紫外线的Norin 1的育种中，对17个水稻品种的祖系和亲缘系进行了UVB敏感性与CPD光解酶活性的相关性研究。在包括Norin 1在内的品种中，CPD光解酶氨基酸序列的第126位氨基酸为精氨酸，其活性较低。该位置的氨基酸为谷氨酰胺，包括佐三石木等品种。此外，抗UVB能力强的品种比抗UVB能力弱的品种表现出更高的光解酶活性。这些结果强调，从谷氨酰胺到精氨酸的单氨基酸变化导致CPD光解酶活性的缺陷，而CPD光解酶活性是决定水稻UVB敏感性的主要因素之一。
There is a cultivar difference in the response to ultraviolet-B (UVB: 280-320 nm) in rice (Oryza sativa L.). Among Japanese lowland rice cultivars, Sasanishiki, a leading Japanese rice cultivar, is resistant to the damaging effects of UVB while Norin 1, a close relative, is less resistant. We found previously that Norin 1 was deficient in cyclobutane pyrimidine dimer (CPD) photorepair ability and suggested that the UVB sensitivity in rice depends largely on CPD photorepair ability. In order to verify that suggestion, we examined the correlation between UVB sensitivity and CPD photolyase activity in 17 rice cultivars of progenitors and relatives in breeding of UV-resistant Sasanishiki and UV-sensitive Norin 1. The amino acid at position 126 of the deduced amino acid sequence of CPD photolyase in cultivars including such as Norin 1 was found to be arginine, the CPD photolyase activities of which were lower. The amino acid at that position in cultivars including such as Sasanishiki was glutamine. Furthermore, cultivars more resistant to UVB were found to exhibit higher photolyase activities than less resistant cultivars. These results emphasize that single amino acid alteration from glutamine to arginine leads to a deficit of CPD photolyase activity and that CPD photolyase activity is one of the main factors determining UVB sensitivity in rice.