Insulin-like growth factors I and II in maternal and fetal guinea pig serum.
Insulin-like growth factors I and II in maternal and fetal guinea pig serum.
复制标题
母体和胎儿豚鼠血清中的胰岛素样生长因子 I 和 II。
作者：
William H. Daughaday;C. E. Yanow;Milan Kapadia
胰岛素样生长因子（IGF）在胎儿发育中的作用一直是很多猜测的主题。我们在豚鼠身上进行了母体和胎儿 IGF I 和 II 的研究，因为妊娠期长且胎儿体型较大，因此可以在比大鼠更长的妊娠和成熟期内进行血液采样。制备胎儿和母体血清的酸性凝胶滤液，用放射免疫分析法测定IGF I； IGF II 通过大鼠胎盘膜放射受体测定法测量。从预计妊娠第 33 天到足月，胎儿 IGF I 水平低于母体水平。妊娠第33天至第49天的胎儿IGF II水平与母体血清的水平没有显着差异[1597 +/- 377 (SE) ng/ml vs. 1295 +/- 224] ng/ml。在妊娠 50 天、55 天和 60 天的胎儿中观察到 IGF II 水平非常高，超过 5000 ng/ml。此后，胎儿 IGF II 水平在足月前显着下降。通过等电聚焦比较怀孕 49 天、50 天、60 天和 65 天后胎儿和母体的 IGF。豚鼠通常有两个主要的 IGF I 基本峰，它们存在于母体和胎儿血清中。大多数母体和胎儿豚鼠血清仅含有单一的、微酸性的 IGF II 峰。我们的方法没有检测到独特的胎儿 IGF 的证据。豚鼠胎儿血清中 IGF II 的水平非常高，表明它可能在胎儿发育中发挥作用。
The role of insulin-like growth factors (IGFs) in fetal development has been the subject of much speculation. We undertook studies of maternal and fetal IGF I and II in the guinea pig because the long gestation period and greater size of the fetuses permitted blood sampling over a longer period of gestation and maturation than is possible in the rat. Acid gel filtrates of fetal and maternal serum were prepared, and the IGF I was measured by RIA; IGF II was measured by rat placental membrane radioreceptor assay. Fetal IGF I levels were lower than maternal levels from the 33rd day of estimated gestation to term. Fetal IGF II levels from the 33rd day to the 49th day of gestation were not significantly different from those of maternal serum [1597 +/- 377 (SE) ng/ml vs. 1295 +/- 224] ng/ml. Very high levels of IGF II, in excess of 5000 ng/ml, were observed in fetuses at 50, 55, and 60 days of gestation. Thereafter, fetal IGF II levels fell markedly before term. Fetal and maternal IGFs after 49, 50, 60, and 65 days of pregnancy were compared by isoelectric focusing. The guinea pig normally has two major basic peaks of IGF I, which were present both in maternal and fetal serum. Most maternal and fetal guinea pig sera contained only a single, slightly acidic peak of IGF II. No evidence of a unique fetal IGF was detected by our methods. The very high levels of IGF II reached in fetal guinea pig sera suggest that it may have a role in fetal development.
非哺乳动物血清的胰岛素样生长因子 I 和 II。
影响因子：
2.7
作者：
Daughaday,WH;Kapadia,M;Yanow,CE;Fabrick,K;Mariz,IK
通讯作者：
Mariz,IK