Detection of active schistosome infection by cell-free circulating DNA of Schistosoma japonicum in highly endemic areas in Sorsogon Province, the Philippines
Detection of active schistosome infection by cell-free circulating DNA of Schistosoma japonicum in highly endemic areas in Sorsogon Province, the Philippines
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DOI：
10.1016/j.actatropica.2014.05.003
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发表时间：
2015-01-01
期刊：
影响因子：
2.7
通讯作者：
Chigusa, Yuichi
中科院分区：
文献类型：
--
作者：
Kato-Hayashi, Naoko;Leonardo, Lydia R.;Chigusa, Yuichi
在血吸虫病高度流行地区，目前的状况是很难确定的卵子检测，因为表面上低寄生虫负荷后，大规模的药物管理，而寄生虫传播率仍然很高。无细胞寄生虫DNA是存在于宿主体液中的寄生虫衍生DNA片段。我们进行了基于人群的研究，以测试存在的无细胞染色体DNA在流行地区的索索贡省，菲律宾。用PCR法检测Kato-Katz（KK）阳性者血清和尿液中的血吸虫DNA（灵敏度为100%）。血吸虫DNA也从KK阴性受试者中检出（9122份血清和10/41份尿液样本）。血吸虫DNA呈网状回声（NW）阳性（血清53.3%，尿42.9%）或NW阴性（血清25.5%，尿20.8%），酶联免疫吸附试验（ELISA）阳性（血清47.1%，尿40%）或ELISA阴性（血清33.3%，尿13.3%）。这些结果表明，在轻度感染的情况下，无细胞染色体DNA是一种有希望的诊断活动性染色体感染的标志物。(C)2014爱思唯尔有限公司版权所有。
The current status of schistosomiasis in highly endemic areas is difficult to determine by ovum detection because of the superficially low parasite load after mass drug administration, whereas the parasite transmission rates are still high. Cell-free parasite DNA is fragments of parasite-derived DNA existing in the host's body fluids. We conducted population-based studies to test the presence of cell-free schistosome DNA in endemic areas of Sorsogon Province, the Philippines. Schistosome DNA in the serum and urine of Kato-Katz (KK)-positive subjects was detected by PCR (100% sensitivity). Schistosome DNA was also detected from KK-negative subjects (9122 serum and 10/41 urine samples). Schistosome DNA was found to be network echogenic pattern (NW)-positive (serum 53.3%, urine 42.9%) or NW-negative (serum 25.5%, urine 20.8%) and enzyme-linked immunosorbent assay (ELISA)-positive (serum 47.1%, urine 40%) or ELISA-negative (serum 33.3%, urine 13.3%). These results indicate that cell-free schistosome DNA is a promising diagnostic marker for active schistosome infection in the case of light infection. (C) 2014 Elsevier B.V. All rights reserved.