Activated monocytes and platelet-monocyte aggregates in patients with sickle cell disease.
Activated monocytes and platelet-monocyte aggregates in patients with sickle cell disease.
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镰状细胞病患者的活化单核细胞和血小板单核细胞聚集体。
DOI：
10.1046/j.1365-2257.2002.00433.x
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Paglieroni,Teresa
中科院分区：
文献类型：
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作者：
Wun,Ted;Cordoba,Miguel;Rangaswami,Arun;Cheung,AnthonyW;Paglieroni,Teresa
肿瘤坏死因子-α（TNF-α）和白细胞介素-1 β（IL-1β）增加内皮表面受体，介导镰状红细胞粘附于内皮。在镰状细胞病（SCD）患者中发现这些细胞因子的循环水平增加。单核细胞是这两种炎症介质的来源;因此，我们确定了循环单核细胞是否在SCD中被激活，如通过这些细胞因子的细胞内表达所定义的。还测定了血液中血小板-单核细胞聚集体（PMA）的存在，因为血小板粘附是单核细胞活化的一种可能机制。SCD患者中表达细胞内TNF-α和IL-1β的单核细胞百分比中位数分别为6.8（2.8-17.3）[中位数（范围）]和14.1（1.3-44.8）。在非裔美国人对照组中，相应的百分比为0.3（0.1-0.5）和0.4（0.1-3.0），白人为0.2（0.1-0.5）和0.8（0.8-1.9）（P<0.001，Kruskal-Wallis）。 白人对照组的PMA平均百分比（±SD）为14.0 ±8.3，非裔美国人对照组为25.7±7.3， SCD患者为45.7±21.6（P< 0.001，RM ANOVA;P<0.05，Newman-Keuls事后检验）。 我们的结论是，有增加循环PMA和单核细胞活化的SCD患者。
Tumour necrosis factor‐α (TNF‐α) and interleukin‐1β (IL‐1β) increase endothelial surface receptors that mediate the adherence of sickle erythrocytes to the endothelium. Increased circulating levels of these cytokines have been found in patients with sickle cell disease (SCD). Monocytes are a source of both of these inflammatory mediators; we therefore determined whether circulating monocytes were activated in SCD, as defined by intracellular expression of these cytokines. Blood was also assayed for the presence of platelet–monocyte aggregates (PMAs), as platelet adherence is one possible mechanism for monocyte activation. The median percentages of monocytes expressing intracellular TNF‐α and IL‐1β in SCD patients were 6.8 (2.8–17.3) [median (range)] and 14.1 (1.3–44.8), respectively. In African‐American controls the corresponding percentages were 0.3 (0.1–0.5) and 0.4 (0.1–3.0), and in Caucasians 0.2 (0.1–0.5) and 0.8 (0.8–1.9) (P< 0.001, Kruskal–Wallis). The mean percentage (± SD) of PMA was 14.0 ± 8.3 for Caucasian controls, 25.7 ± 7.3 for African‐American controls, and 45.7 ± 21.6 for patients with SCD (P< 0.001, RM ANOVA;P< 0.05, Newman–Keuls posthoc test). We conclude that there are increased circulating PMAs and monocyte activation in patients with SCD.