Semiautomated method for the quantitation of plasma or sera androstenedione, testosterone, and dihydrotestosterone in population studies.
Semiautomated method for the quantitation of plasma or sera androstenedione, testosterone, and dihydrotestosterone in population studies.
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群体研究中血浆或血清雄烯二酮、睾酮和二氢睾酮定量的半自动方法。
DOI：
10.1006/abio.1996.0072
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发表时间：
1996
影响因子：
2.9
通讯作者：
Schroeder,RR
中科院分区：
文献类型：
--
作者：
Walker,VR;Dombi,GW;Gutai,JP;Wade,DD;Swartz,KH;Liu,H;Schroeder,RR
提出了一种方法，定量和重复性分析雄激素睾酮，雄烯二酮，和双氢睾酮从人血清或血浆。色谱分离步骤产生每小时一次制备分离的无人值守通量。方法中内置了对照品，以说明色谱条件的变化，否则会导致保留特性发生变化。三种雄激素的分离因子如下（平均值±标准差）：雄烯二酮和睾酮之间的α = 1.23 ± 0.011，睾酮和双氢睾酮之间的α = 1.38 ± 0.025。该方法的灵敏度为雄烯二酮5 pg，睾酮3 pg，和双氢睾酮14 pg。与初始样品处理，验证，并应用于两个样本集，这表明该方法的实用性分析的低雄激素人群（绝经后妇女）和高雄激素组（前列腺癌患者）的程序损失的研究也有报道。对照血浆重复等分试样的精密度为雄烯二酮和睾酮= 5-11% CV，双氢睾酮= 10-20% CV。
A method is presented that analyzes quantitatively and reproducibly the androgens testosterone, androstenedione, and dihydrotestosterone from human sera or plasma. The chromatographic separation step generates an unattended throughput of one preparative separation per hour. Controls are built into the method to account for changing chromatographic conditions that otherwise result in shifts in retention characteristics. Separation factors for the three androgens are as follows (mean ± standard deviation): α = 1.23 ± 0.011 between androstenedione and testosterone and α = 1.38 ± 0.025 between testosterone and dihydrotestosterone. Sensitivities of the method are androstenedione 5 pg, testosterone 3 pg, and dihydrotestosterone 14 pg. A study of procedural losses associated with initial sample processing, a validation, and application to two sample sets which demonstrates the methods utility for the analysis of hypoandrogenic populations (postmenopausal women) and hyperandrogenic groups (prostate cancer patients) is also reported. The precision for replicate aliquots of control plasma is androstenedione and testosterone = 5–11% CV and dihydrotestosterone = 10–20% CV.