Spo0A regulates chromosome copy number during sporulation by directly binding to the origin of replication in Bacillus subtilis
Spo0A regulates chromosome copy number during sporulation by directly binding to the origin of replication in Bacillus subtilis
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DOI：
10.1111/mmi.12141
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发表时间：
2013-02-01
影响因子：
3.6
通讯作者：
Veening, Jan-Willem
中科院分区：
文献类型：
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作者：
Boonstra, Mirjam;de Jong, Imke G.;Veening, Jan-Willem
在饥饿状态下，枯草芽孢杆菌细胞可以通过激活主转录调控因子Spo0A进入内孢子形成的发育程序。正确的染色体拷贝数对生产成熟和完全抗性的孢子至关重要。母细胞的一条染色体和前孢子的一份拷贝的产生和维持需要DNA复制和孢子形成的启动之间的准确协调。在这里，我们证明了Spo0A通过直接与复制起点(ORIC)附近存在的一些Spo0A结合位点来调节染色体拷贝数。我们证明，在ORIC上缺乏三个特定Spo0A结合位点的细胞在诱导产孢子时会增加染色体拷贝数。我们的数据支持这样的假设，即Spo0A通过与ORIC结合来直接控制孢子形成过程中的DNA复制。
When starved, Bacillus subtilis cells can enter the developmental programme of endospore formation by activation of the master transcriptional regulator Spo0A. Correct chromosome copy number is crucial for the production of mature and fully resistant spores. The production and maintenance of one chromosome for the mother cell and one copy for the forespore requires accurate co-ordination between DNA replication and initiation of sporulation. Here, we show that Spo0A regulates chromosome copy number by directly binding to a number of Spo0A binding sites that are present near the origin of replication (oriC). We demonstrate that cells lacking three specific Spo0A binding sites at oriC display increased chromosome copy numbers when sporulation is induced. Our data support the hypothesis that Spo0A directly controls DNA replication during sporulation by binding to oriC.