Medicinal and Edible Lignicolous Fungi as Natural Sources of Antioxidative and Antibacterial Agents
Medicinal and Edible Lignicolous Fungi as Natural Sources of Antioxidative and Antibacterial Agents
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DOI：
10.1002/ptr.2969
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发表时间：
2010-10-01
影响因子：
7.2
通讯作者：
Popovic, M.
中科院分区：
文献类型：
--
作者：
Karaman, M.;Jovin, E.;Popovic, M.
以清除DPPH中心点和OH中心点自由基能力、抗脂质过氧化能力和抑菌活性为指标，研究了灵芝、树舌灵芝、灰树花、硫磺菌、金针菇、云芝、糙皮侧耳和虎斑革耳菌8种真菌有机提取物的抗氧化活性，并采用琼脂扩散法测定了其抑菌活性。结果表明，甘草甲醇提取物清除DPPH中心点的活性最高。applanatum（12.5 μ g/mL，82.80%）和G.灵芝（510.2 μ g/mL，69.12%）。相同的提取物在500 μ g/mL时也表现出最高的LP抑制率（91.83%，85.09%），而G. applanatum和L.在400 μ g/mL时，硫磺菌对OH中心点自由基的清除率最高，分别为68.47%，57.06%。对革兰氏阳性菌也表现出较强的抗菌活性。抗氧化能力与总酚含量（0.19-9.98 mg/g）相关。HPLC测定表明，大多数分析的物种含有没食子酸和原儿茶酸。因此，这些真菌被证明是抗氧化剂和抗菌剂的潜在来源。版权所有（c）2010约翰威利父子有限公司。
The antioxidant activity of organic extracts of eight fungal species, Ganoderma lucidum, Ganoderma applanatum, Meripilus giganteus, Laetiporus sulphureus, Flammulina velutipes, Coriolus versicolor, Pleurotus ostreatus and Panus tigrinus, was evaluated for free radical (DPPH center dot and OH center dot) scavenging capacity and an effect on lipid peroxidation, and the antibacterial activity was tested by the agar well diffusion method. The highest DPPH center dot scavenging activity was found in the methanol extract of G. applanatum (12.5 mu g/mL, 82.80%) and the chloroform extract of G. lucidum (510.2 mu g/mL, 69.12%). The same extracts also showed the highest LP inhibition (91.83%, 85.09%) at 500 mu g/mL, while the methanol extracts of G. applanatum and L. sulphureus showed the highest scavenging effect on OH center dot radicals (68.47%, 57.06%, respectively) at 400 mu g/mL. A strong antibacterial activity against Gram-positive bacteria was also manifested. The antioxidative potencies correlated generally with the total phenol content (0.19-9.98 mg/g). The HPLC determination showed that the majority of analysed species contained gallic and protocatechic acids. Consequently, these fungi are shown to be potential sources of antioxidative and antibacterial agents. Copyright (c) 2010 John Wiley & Sons, Ltd.