Intracellular nanoparticles mass quantification by near-edge absorption soft X-ray nanotomography.
Intracellular nanoparticles mass quantification by near-edge absorption soft X-ray nanotomography.
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DOI：
10.1038/srep22354
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发表时间：
2016-03-10
影响因子：
4.6
通讯作者：
Carrascosa JL
中科院分区：
文献类型：
--
作者：
Conesa JJ;Otón J;Chiappi M;Carazo JM;Pereiro E;Chichón FJ;Carrascosa JL
我们使用软X射线三维成像技术，通过利用氧化铁的差示吸收对比度，对整个细胞内的超顺磁性氧化铁纳米颗粒(SPION)的质量进行了量化。近边吸收软X射线纳米层析成像结合了50 nm分辨率的全细胞三维结构测定、三维元素映射和高通量。我们检测到SPION在细胞内的三维分布，灵敏度为0.3cmg/ ，足以检测对应于单个纳米颗粒的密度。
We used soft X-ray three-dimensional imaging to quantify the mass of superparamagnetic iron oxide nanoparticles (SPION) within whole cells, by exploiting the iron oxide differential absorption contrast. Near-edge absorption soft X-ray nanotomography (NEASXT) combines whole-cell 3D structure determination at 50 nm resolution, with 3D elemental mapping and high throughput. We detected three-dimensional distribution of SPIONs within cells with 0.3 g/cm3 sensitivity, sufficient for detecting the density corresponding to a single nanoparticle.