Quasi-elastic light scattering studies of native hepatic bile from the dog: comparison with aggregative behavior of model biliary lipid systems.
Quasi-elastic light scattering studies of native hepatic bile from the dog: comparison with aggregative behavior of model biliary lipid systems.
复制标题
狗天然肝胆汁的准弹性光散射研究：与模型胆汁脂质系统的聚合行为进行比较。
DOI：
10.1021/bi00304a018
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Känzig,W
中科院分区：
文献类型：
--
作者：
Mazer,NA;Schurtenberg,P;Carey,MC;Preisig,R;Weigand,K;Känzig,W
N. A. Mazer，* P. Schurtenberger，M. C.凯里河Preisig，K. Weigand和W. Kanzig摘要：本文用准弹性光散射（QLS）方法对狗肝胆汁中的大分子组分进行了表征，并与含有胆盐（BS）、牛磺胆酸钠（TC）、卵磷脂（L）和胆固醇（Ch）的模型胆汁溶液的数据进行了比较。通过在先前胆囊切除的犬中直接插入胆总管导管（配有托马斯十二指肠插管）获得天然胆汁样本。在三种分泌状态下对肝胆汁进行采样：（A）未刺激的“空腹”胆汁，（B）静脉内TC输注期间的“刺激”分泌，和（C）“促分泌素刺激”分泌。三个样本的L/BS和Ch/L的摩尔比相当（分别为0.21±0.03和0.027±0.006），但总脂质浓度（BS+ L+ Ch）不同：（A）13.1±0.8，（B）6.7±0.8，（C）3.0±0.4 g/dL。从对样品B和C测量的QLS自相关函数中，分辨出三种大分子组分（表示为1/3和2）。组分Ia（流体动力学半径Ria= 10±2 μ m）的大小与模型体系的胶束聚集体相当。组分1/3（Rtp= 67±7 μ g）似乎反映了胆汁蛋白的平均值。组分2（R2= 650±15 μ m）为痕量组分，其大小和沉积行为与组分1（R2 = 650±15 μ m）相似。
N. A. Mazer,* P. Schurtenberger, M. C. Carey, R. Preisig, K. Weigand, and W. Kanzig abstract: Using quasi-elastic light scattering (QLS), we have characterized the macromolecular components in hepatic bile obtained from the dog and comparedthese results with data from model bilesolutions containing the bile salt (BS) sodium taurocholate (TC), egg lecithin (L), and cholesterol (Ch). Native bile samples were obtained by direct catheterization of the common bile duct in a previously cholecystectomized dog fitted with a Thomas duodenal cannula. Hepatic bile was sampled during three secretory states:(A) unstimulated “fasting” bile,(B)“stimulated” secretion during an intravenous TC infusion, and (C)“secretin-stimulated” secretion. All three samples had comparablemolar ratios of L/BS (0.21±0.03) and Ch/L (0.027±0.006) but differed in the totallipid concentration (BS+ L+ Ch):(A) 13.1±0.8,(B) 6.7±0.8, and (C) 3.0±0.4 g/dL. From the QLS autocorrelation functions measured on samples B and C, three macromolecular components (denoted, 1/3, and 2) were resolved. Component la (hydrodynamic radius Ria= 10±2 Á) is comparable in size tothe micellar aggregates of model systems. Com-ponent 1/3 (Rtp= 67±7 Á) appears to reflect an average of biliary proteins. Component 2 {R2= 650±15 Á) is a trace component whose size and sedimentation behavior are com-