HIGHER-ORDER NUCLEAR-STRUCTURE IN MAMMALIAN SPERM REVEALED BY IN-SITU HYBRIDIZATION AND EXTENDED CHROMATIN FIBERS
HIGHER-ORDER NUCLEAR-STRUCTURE IN MAMMALIAN SPERM REVEALED BY IN-SITU HYBRIDIZATION AND EXTENDED CHROMATIN FIBERS
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DOI：
10.1006/excr.1995.1270
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发表时间：
1995-08-01
影响因子：
3.7
通讯作者：
WARD, DC
中科院分区：
文献类型：
--
作者：
HAAF, T;WARD, DC
人类和小鼠精子细胞核中染色体的高级组织已通过荧光原位杂交显现出来。在小鼠睾丸精子中，着丝粒形成几个线性高级结构，这些结构附着在细胞核中心的异染色质中心上。近端着丝粒小鼠染色体的端粒与异染色质中心或核膜相关。整个染色体结构域平行于异染色质中心排列，有时缠绕在其周围。我们建议组成型异染色质作为小鼠精子染色质细胞类型特异性组织的成核点。在人类成熟的精子中，单个染色体也表现为拉长的区域。当用高盐和洗涤剂裂解人类精子细胞核时，正常浓缩的精子染色质会分解成线性阵列，呈现出离散的结节状亚结构（
The higher order organization of chromosomes in human and mouse sperm-cell nuclei has been visualized by fluorescence in situ hybridization. In mouse testicular sperm, centromeres form several linear higher order structures that are attached to a heterochromatic chromocenter at the center of the nucleus. Telomeres of the acrocentric mouse chromosomes are associated with either the heterochromatic center or the nuclear membrane. Whole chromosome domains are arranged parallel to the heterochromatic chromocenter and occasionally wrapped around it. We propose that constitutive heterochromatin serves as the nucleation point for a cell-type-specific organization of mouse sperm chromatin. In human mature sperm, individual chromosomes also appear as elongated territories. When human sperm nuclei are lysed with high salt and detergent, the normally condensed sperm chromatin unravels into linear arrays that exhibit a discrete nodular substructure (of