Quantitative assessment of optical clearing methods in various intact mouse organs
Quantitative assessment of optical clearing methods in various intact mouse organs
复制标题
各种完整小鼠器官中光学清除方法的定量评估
DOI：
10.1002/jbio.201800134
复制
发表时间：
2019
影响因子：
2.8
通讯作者：
Zhu Dan
中科院分区：
文献类型：
--
作者：
Xu Jianyi;Ma Yilin;Yu Tingting;Zhu Dan
Various tissue optical clearing techniques have sprung up for large volume imaging. However, there are few methods showed clearing and imaging data on different organs while most of them were focused on mouse brain, and as a result, it is difficult to select the suitable method for organs in practical applications due to lack of quantitative evaluation and comprehensive comparison. Therefore, it is necessary to evaluate and compare the performances of clearing methods for different organs. In this paper, several typical optical clearing methods were applied, including 3DISCO, uDISCO, SeeDB, FRUIT, CUBIC, ScaleS and PACT to clear intact brain, heart, kidney, liver, spleen, stomach, lung, small intestine, skin and muscle. The clearing efficiency, sample deformation, fluorescence preservation and imaging depth of these methods were quantitatively evaluated. Finally, based on the systemic evaluation of various parameters described above, the appropriate clearing method for specific organ including kidney or intestine was screened out. This paper will provide important references for selection of appropriate clearing methods in related researches.
