Methods for Studying Movement of Molecules Within Cilia.
Methods for Studying Movement of Molecules Within Cilia.
复制标题
研究纤毛内分子运动的方法。
DOI：
10.1007/978-1-4939-3789-9_6
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Lechtreck KF
中科院分区：
文献类型：
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作者：
Lechtreck KF
纤毛和真核鞭毛(可互换术语)的组装需要将大量蛋白质从细胞体输入到生长的细胞器中。蛋白质通过扩散和基于马达的鞭毛内转运(IFT)进入纤毛和纤毛内部。纤毛蛋白转运的许多方面，如卸载部位的分布和转运的频率，可以使用荧光标记蛋白的直接活体成像进行分析。在这里，我们将描述如何使用全内反射荧光显微镜(TIRFM)来分析单细胞藻莱茵衣藻鞭毛中的蛋白质运输，这是一种广泛使用的纤毛和纤毛相关疾病的模型。
The assembly of cilia and eukaryotic flagella (interchangeable terms) requires the import of numerous proteins from the cell body into the growing organelle. Proteins move into and inside cilia by diffusion and by motor-based intraflagellar transport (IFT). Many aspects of ciliary protein transport such as the distribution of unloading sites and the frequency of transport can be analyzed using direct in vivo imaging of fluorescently tagged proteins. Here, we will describe how to use total internal reflection fluorescence microcopy (TIRFM) to analyze protein transport in flagella using in the unicellular alga Chlamydomonas reinhardtii, a widely used model for cilia and cilia-related disease.