CHLORAMPHENICOL‐ AND CYCLOHEXIMIDE‐SENSITIVE PROTEIN SYNTHETIC SYSTEMS IN BRAIN MITOCHONDRIAL AND NERVE‐ENDING PREPARATIONS
CHLORAMPHENICOL‐ AND CYCLOHEXIMIDE‐SENSITIVE PROTEIN SYNTHETIC SYSTEMS IN BRAIN MITOCHONDRIAL AND NERVE‐ENDING PREPARATIONS
复制标题
脑线粒体和神经末梢制剂中的氯霉素和放线菌酮敏感蛋白合成系统
DOI：
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发表时间：
1973
影响因子：
4.7
通讯作者：
M. W. Gordon
中科院分区：
文献类型：
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作者：
G. G. Deanin;M. W. Gordon
摘要-从大鼠脑中分离的线粒体含有核糖体，其表观沉降常数（j）为60，可分解为45 s和32 s亚基。与45 s亚基相关的RNA的s值为16; 32 s亚基的RNA的s值为13。这些值基本上与大鼠肝脏线粒体中发现的值相似。与60年代单体相关的新生蛋白质被氯霉素减少，但不被环己酰亚胺减少。在由蔗糖或Ficoll-蔗糖梯度制备的脑线粒体中发现的所有放线菌酮敏感活性以及与类似制备的神经末梢制剂相关的大部分活性可归因于污染物，该污染物可能是一种膜封闭的细胞质样系统，除了18 s和28 s RNA外，还含有一个或多个线粒体。已去除大部分或全部污染的神经末梢制剂显示出非常少的放线菌酮敏感蛋白合成活性。
Abstract— Mitochondria isolated from rat brain contained ribosomes, with an apparent sedimentation constant (j) of 60, which were dissociable into 45s and 32s subunits. The RNA associated with the 45s subunit had an s value of 16; the RNA of the 32s subunit had an s value of 13. These values were essentially like those found for the mitochondria of rat liver. Nascent protein associated with the 60s monosome was reduced by chloramphenicol but not by cycloheximide. All cycloheximide‐sensitive activity found in brain mitochondria prepared from sucrose or Ficoll‐sucrose gradients and most of that associated with nerve‐ending preparations that were similarly prepared could be attributed to a contaminant that was probably a membrane‐enclosed, cytoplasmic‐like system which contained, in addition to 18s and 28s RNA, one or more mitochondria. Nerve‐ending preparations from which most or all of this contamination had been removed exhibited very little cycloheximide‐sensitive protein synthetic activity.