Cell Microencapsulation Within Engineered Hyaluronan Elastin-Like Protein (HELP) Hydrogels.
Cell Microencapsulation Within Engineered Hyaluronan Elastin-Like Protein (HELP) Hydrogels.
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工程透明质酸弹性蛋白样蛋白 (HELP) 水凝胶内的细胞微囊化。
DOI：
10.1002/cpz1.917
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Heilshorn,SarahC
中科院分区：
文献类型：
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作者：
Hefferon,MeghanE;Huang,MichelleS;Liu,Yueming;Navarro,RenatoS;dePaivaNarciso,Narelli;Zhang,Daiyao;Aviles-Rodriguez,Giselle;Heilshorn,SarahC
三维细胞包封已经使其本身成为组织工程领域的一个钉。使用重组工程化的、基于生物聚合物的水凝胶来封装细胞是特别有前途的，因为它提供了增强的控制和可调性。在这里，我们详细描述了我们的透明质酸（即，透明质酸）和弹性蛋白样蛋白（HELP）水凝胶系统。除了验证我们的合成过程的有效性，我们还展示了HELP系统的模块化。最后，我们表明，细胞可以封装在HELP凝胶在一定范围内的刚度，表现出很强的活力，并响应刚度线索。© 2023 Wiley Periodicals LLC.基本方案1：用肼修饰弹性蛋白样蛋白基本方案2：用肼修饰弹性蛋白样蛋白的核磁共振定量基本方案3：透明质酸-苯甲醛合成基本方案4：透明质酸-苯甲醛的核磁共振定量基本方案5：透明质酸弹性蛋白样蛋白凝胶中的3D细胞包封
Three‐dimensional cell encapsulation has rendered itself a staple in the tissue engineering field. Using recombinantly engineered, biopolymer‐based hydrogels to encapsulate cells is especially promising due to the enhanced control and tunability it affords. Here, we describe in detail the synthesis of our hyaluronan (i.e., hyaluronic acid) and elastin‐like protein (HELP) hydrogel system. In addition to validating the efficacy of our synthetic process, we also demonstrate the modularity of the HELP system. Finally, we show that cells can be encapsulated within HELP gels over a range of stiffnesses, exhibit strong viability, and respond to stiffness cues. © 2023 Wiley Periodicals LLC.Basic Protocol 1: Elastin‐like protein modification with hydrazineBasic Protocol 2: Nuclear magnetic resonance quantification of elastin‐like protein modification with hydrazineBasic Protocol 3: Hyaluronic acid–benzaldehyde synthesisBasic Protocol 4: Nuclear magnetic resonance quantification of hyaluronic acid–benzaldehydeBasic Protocol 5: 3D cell encapsulation in hyaluronan elastin‐like protein gels