Notch signaling functions as a binary switch for the determination of glandular and luminal fates of endodermal epithelium during chicken stomach development
Notch signaling functions as a binary switch for the determination of glandular and luminal fates of endodermal epithelium during chicken stomach development
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DOI：
10.1242/dev.01853
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发表时间：
2005-06-01
期刊：
影响因子：
4.6
通讯作者：
Yasugi, S
中科院分区：
文献类型：
--
作者：
Matsuda, Y;Wakamatsu, Y;Yasugi, S
在鸡的腺胃(腺胃)发育过程中，肠道内胚层分化为腺上皮和腔上皮。我们发现，在腺体形成的早期，Delta1表达细胞、未分化细胞和Notch激活的细胞共存于内皮细胞内。用Numb或显性阴性的SU(H)抑制Notch信号导致腔分化，而强制激活Notch信号则促进未成熟腺细胞的规范，但阻止随后的分化和腺体内陷。这些结果表明，Delta1介导的内皮细胞间的Notch信号是决定腺体和腔细胞命运的二元开关，并调节鸡腺胃中腺体的模式分化。
During development of the chicken proventriculus (glandular stomach), gut endoderm differentiates into glandular and luminal epithelium. We found that Delta1 expressing cells, undifferentiated cells and Notch-activated cells colocalize within the endodermal epithelium during early gland formation. Inhibition of Notch signaling using Numb or dominant-negative form of Su(H) resulted in a luminal differentiation, while forced activation of Notch signaling promoted the specification of immature glandular cells, but prevented the subsequent differentiation and the invagination of the glands. These results suggest that Delta1-mediated Notch signaling among endodermal cells functions as a binary switch for determination of glandular and luminal fates, and regulates patterned differentiation of glands in the chicken proventriculus.