Protective mechanisms of resveratrol against ischemia-reperfusion-induced damage in hearts obtained from Zucker obese rats: the role of GLUT-4 and endothelin
Protective mechanisms of resveratrol against ischemia-reperfusion-induced damage in hearts obtained from Zucker obese rats: the role of GLUT-4 and endothelin
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DOI：
10.1152/ajpheart.01048.2007
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发表时间：
2008-02-01
影响因子：
4.8
通讯作者：
Tosaki, Arpad
中科院分区：
文献类型：
--
作者：
Lekli, Istvan;Szabo, Gergo;Tosaki, Arpad
在Zucker肥胖大鼠中研究白藜芦醇诱导的心脏保护作用。将大鼠分为5组：第1组，瘦对照组;第2组，肥胖对照组（OC）;第3组，肥胖大鼠mg.kg藜芦醇（OR）5天，持续2周;第4组，肥胖大鼠自由进食10%葡萄糖溶液，持续3周（OG）;第5组，肥胖大鼠接受10%葡萄糖，持续3周，并在第2和第3周期间接受白藜芦醇（OGR）。测量体重、血清葡萄糖和胰岛素，然后分离心脏并进行30分钟的缺血，随后进行120分钟的再灌注。测量心率、冠状动脉血流量、主动脉血流量、发展压力、再灌注诱导的室颤发生率和梗死面积。与OC值相比，白藜芦醇降低了OR中的体重和血清葡萄糖（分别为414 +/- 10 g和7.08 +/- 0.41 mmol/l，至378 +/- 12 g和6.11 +/- 0.44 mmol/l），但胰岛素水平不变。OG与OGR组获得了相同的结果。与无白藜芦醇组相比，白藜芦醇在有或无葡萄糖摄入的情况下改善缺血后心脏功能。与OC和OG组相比，OR组室颤和梗死面积的发生率分别降低了83%和20%，OGR组降低了67%和16%。白藜芦醇增加GLUT-4的表达，减少内皮素的表达和心肌细胞凋亡在缺血再灌注心脏在存在或不存在葡萄糖摄入。因此，白藜芦醇的保护作用可能与其对心脏的直接影响有关。
The resveratrol-induced cardiac protection was studied in Zucker obese rats. Rats were divided into five groups: group 1, lean control; group 2, obese control (OC); group 3, obese rats treated orally with 5 mg.kg(-1).day(-1) of resveratrol (OR) for 2 wk; group 4, obese rats received 10% glucose solution ad libitum for 3 wk (OG); and group 5, obese rats received 10% glucose for 3 wk and resveratrol (OGR) during the 2nd and 3rd wk. Body weight, serum glucose, and insulin were measured, and then hearts were isolated and subjected to 30 min of ischemia followed by 120 min of reperfusion. Heart rate, coronary flow, aortic flow, developed pressure, the incidence of reperfusion-induced ventricular fibrillation, and infarct size were measured. Resveratrol reduced body weight and serum glucose in the OR compared with the OC values (414 +/- 10 g and 7.08 +/- 0.41 mmol/l, respectively, to 378 +/- 12 g and 6.11 +/- 0.44 mmol/l), but insulin levels were unchanged. The same results were obtained for the OG vs. OGR group. Resveratrol improved postischemic cardiac function in the presence or absence of glucose intake compared with the resveratrol-free group. The incidence of ventricular fibrillation and infarct size was reduced by 83 and 20% in the OR group, and 67 and 16% in the OGR group, compared with the OC and OG groups, respectively. Resveratrol increased GLUT-4 expression and reduced endothelin expression and cardiac apoptosis in ischemic-reperfused hearts in the presence or absence of glucose intake. Thus the protective effect of resveratrol could be related to its direct effects on the heart.